Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12390 | 3' | -52.7 | NC_003324.1 | + | 26871 | 0.66 | 0.805543 |
Target: 5'- uUUUCAGGCUCAAGGGUGAcGccGGGc -3' miRNA: 3'- uAGAGUUCGAGUUUCCGUUcCcuCCC- -5' |
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12390 | 3' | -52.7 | NC_003324.1 | + | 47659 | 0.66 | 0.834074 |
Target: 5'- -cUUCcGGCUCGAcGGCGAGGGucGa -3' miRNA: 3'- uaGAGuUCGAGUUuCCGUUCCCucCc -5' |
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12390 | 3' | -52.7 | NC_003324.1 | + | 27385 | 0.69 | 0.67735 |
Target: 5'- uGUCgcc-GCacgCGAAGGCAAGGGGcGGGa -3' miRNA: 3'- -UAGaguuCGa--GUUUCCGUUCCCU-CCC- -5' |
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12390 | 3' | -52.7 | NC_003324.1 | + | 45061 | 1.08 | 0.001799 |
Target: 5'- uAUCUCAAGCUCAAAGGCAAGGGAGGGu -3' miRNA: 3'- -UAGAGUUCGAGUUUCCGUUCCCUCCC- -5' |
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12390 | 3' | -52.7 | NC_003324.1 | + | 57396 | 0.69 | 0.666078 |
Target: 5'- -cCUCAcugcuggagGGCUCGgcgGAGGCGAGGGcGGc -3' miRNA: 3'- uaGAGU---------UCGAGU---UUCCGUUCCCuCCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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