Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12391 | 3' | -54.9 | NC_003324.1 | + | 7316 | 0.66 | 0.713994 |
Target: 5'- -aCCgugACcgUCGuuccGCGCGCCgaGGGCACGc -3' miRNA: 3'- caGGa--UGa-AGCu---UGCGCGG--UCCGUGU- -5' |
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12391 | 3' | -54.9 | NC_003324.1 | + | 8184 | 0.69 | 0.559937 |
Target: 5'- -cCCUGCUcacugcccggCGGcGCGCGCCuGGCACu -3' miRNA: 3'- caGGAUGAa---------GCU-UGCGCGGuCCGUGu -5' |
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12391 | 3' | -54.9 | NC_003324.1 | + | 11455 | 0.7 | 0.495932 |
Target: 5'- cUCC-ACUg-GAACGCGCagGGGCACAu -3' miRNA: 3'- cAGGaUGAagCUUGCGCGg-UCCGUGU- -5' |
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12391 | 3' | -54.9 | NC_003324.1 | + | 11842 | 0.79 | 0.148892 |
Target: 5'- uGUCCUGCg-CGAcCGgGCCGGGCACGg -3' miRNA: 3'- -CAGGAUGaaGCUuGCgCGGUCCGUGU- -5' |
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12391 | 3' | -54.9 | NC_003324.1 | + | 13855 | 0.67 | 0.635998 |
Target: 5'- aGUUUUgGCUUCGugcgggagggcguAAUGCGCCAGGCAg- -3' miRNA: 3'- -CAGGA-UGAAGC-------------UUGCGCGGUCCGUgu -5' |
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12391 | 3' | -54.9 | NC_003324.1 | + | 13968 | 0.66 | 0.713994 |
Target: 5'- gGUCCUcgcCUUCGuGACcUGCCuGGCGCAu -3' miRNA: 3'- -CAGGAu--GAAGC-UUGcGCGGuCCGUGU- -5' |
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12391 | 3' | -54.9 | NC_003324.1 | + | 15743 | 0.66 | 0.735396 |
Target: 5'- -gCCUugaucGCgucaUCGAGCGCGCCGGGg--- -3' miRNA: 3'- caGGA-----UGa---AGCUUGCGCGGUCCgugu -5' |
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12391 | 3' | -54.9 | NC_003324.1 | + | 16123 | 0.75 | 0.26184 |
Target: 5'- gGUCCUgcgcagggcGCUUCGGuagcgggggcCGCGCCAGGCAUc -3' miRNA: 3'- -CAGGA---------UGAAGCUu---------GCGCGGUCCGUGu -5' |
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12391 | 3' | -54.9 | NC_003324.1 | + | 19018 | 0.66 | 0.745944 |
Target: 5'- -gCUgGCcUCGAACGCGaugcgCAGGCGCGu -3' miRNA: 3'- caGGaUGaAGCUUGCGCg----GUCCGUGU- -5' |
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12391 | 3' | -54.9 | NC_003324.1 | + | 21584 | 0.67 | 0.681306 |
Target: 5'- cGUCCgACcUUGAgcaACGCGaCGGGCGCGg -3' miRNA: 3'- -CAGGaUGaAGCU---UGCGCgGUCCGUGU- -5' |
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12391 | 3' | -54.9 | NC_003324.1 | + | 23657 | 0.74 | 0.290003 |
Target: 5'- aGUCCUcgauauGCUUCGccgguauccacGGCGCGCCGGGCuucACAu -3' miRNA: 3'- -CAGGA------UGAAGC-----------UUGCGCGGUCCG---UGU- -5' |
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12391 | 3' | -54.9 | NC_003324.1 | + | 26007 | 0.66 | 0.735396 |
Target: 5'- -cCCgACUUCGGggugacugaGCGCGCCAauGGCGuCAc -3' miRNA: 3'- caGGaUGAAGCU---------UGCGCGGU--CCGU-GU- -5' |
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12391 | 3' | -54.9 | NC_003324.1 | + | 28932 | 0.74 | 0.297405 |
Target: 5'- cUCCUGCUUUG-GCGUGCCGccuGGCGCu -3' miRNA: 3'- cAGGAUGAAGCuUGCGCGGU---CCGUGu -5' |
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12391 | 3' | -54.9 | NC_003324.1 | + | 29477 | 0.78 | 0.15308 |
Target: 5'- -gCCUgGCUgCGGGCGCGUCGGGCGCAu -3' miRNA: 3'- caGGA-UGAaGCUUGCGCGGUCCGUGU- -5' |
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12391 | 3' | -54.9 | NC_003324.1 | + | 31213 | 0.68 | 0.626019 |
Target: 5'- -gCCUGCa------GCGCCAGGCGCAa -3' miRNA: 3'- caGGAUGaagcuugCGCGGUCCGUGU- -5' |
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12391 | 3' | -54.9 | NC_003324.1 | + | 31959 | 0.66 | 0.703165 |
Target: 5'- uGUCCggagcgCGAAgcccagaGCGUCAGGCGCAg -3' miRNA: 3'- -CAGGaugaa-GCUUg------CGCGGUCCGUGU- -5' |
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12391 | 3' | -54.9 | NC_003324.1 | + | 37422 | 0.67 | 0.692265 |
Target: 5'- cGUUCUugUugcuaUCGAGCGCauuGCCGcGCACAg -3' miRNA: 3'- -CAGGAugA-----AGCUUGCG---CGGUcCGUGU- -5' |
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12391 | 3' | -54.9 | NC_003324.1 | + | 42308 | 0.66 | 0.735396 |
Target: 5'- -gCCUGCc----ACGUGCCGGGCGCc -3' miRNA: 3'- caGGAUGaagcuUGCGCGGUCCGUGu -5' |
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12391 | 3' | -54.9 | NC_003324.1 | + | 42618 | 0.71 | 0.445235 |
Target: 5'- -gUCUGCUuauagucaUCGcGCgGCGCCGGGCGCGg -3' miRNA: 3'- caGGAUGA--------AGCuUG-CGCGGUCCGUGU- -5' |
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12391 | 3' | -54.9 | NC_003324.1 | + | 45237 | 1.09 | 0.001115 |
Target: 5'- cGUCCUACUUCGAACGCGCCAGGCACAu -3' miRNA: 3'- -CAGGAUGAAGCUUGCGCGGUCCGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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