Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12391 | 5' | -59.4 | NC_003324.1 | + | 20174 | 0.69 | 0.319978 |
Target: 5'- --cGCuGCCGGCAGCcucGGUCaGAGAGgCg -3' miRNA: 3'- cuaUG-CGGCCGUCG---CCAG-CUCUCgGa -5' |
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12391 | 5' | -59.4 | NC_003324.1 | + | 44891 | 0.68 | 0.403322 |
Target: 5'- cGGUACGCCGG-GGCG--CGGGGGCUg -3' miRNA: 3'- -CUAUGCGGCCgUCGCcaGCUCUCGGa -5' |
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12391 | 5' | -59.4 | NC_003324.1 | + | 53465 | 0.67 | 0.449835 |
Target: 5'- --aACGCCGGCAaguacacccgcGCGGaagcCGAG-GCCg -3' miRNA: 3'- cuaUGCGGCCGU-----------CGCCa---GCUCuCGGa -5' |
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12391 | 5' | -59.4 | NC_003324.1 | + | 18020 | 0.67 | 0.453683 |
Target: 5'- cGAUugGCCGGCgccAcgggccaugcgauagGCGG-CGAGuGCCg -3' miRNA: 3'- -CUAugCGGCCG---U---------------CGCCaGCUCuCGGa -5' |
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12391 | 5' | -59.4 | NC_003324.1 | + | 35322 | 0.66 | 0.489088 |
Target: 5'- -uUGCGCCGGCugcuGCGGUUGcuguuGCUg -3' miRNA: 3'- cuAUGCGGCCGu---CGCCAGCucu--CGGa -5' |
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12391 | 5' | -59.4 | NC_003324.1 | + | 47910 | 0.66 | 0.489088 |
Target: 5'- cGAUG-GCCGGCuuauccGCugGGUCGAGAGUUUc -3' miRNA: 3'- -CUAUgCGGCCGu-----CG--CCAGCUCUCGGA- -5' |
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12391 | 5' | -59.4 | NC_003324.1 | + | 4847 | 0.7 | 0.276506 |
Target: 5'- --gGCGCCGGCAacaacguuGCGGcCGGucGAGCCc -3' miRNA: 3'- cuaUGCGGCCGU--------CGCCaGCU--CUCGGa -5' |
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12391 | 5' | -59.4 | NC_003324.1 | + | 45203 | 1.07 | 0.000525 |
Target: 5'- uGAUACGCCGGCAGCGGUCGAGAGCCUg -3' miRNA: 3'- -CUAUGCGGCCGUCGCCAGCUCUCGGA- -5' |
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12391 | 5' | -59.4 | NC_003324.1 | + | 13167 | 0.77 | 0.088104 |
Target: 5'- -cUGCGUCGGCGGCGGUCGGcGGCg- -3' miRNA: 3'- cuAUGCGGCCGUCGCCAGCUcUCGga -5' |
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12391 | 5' | -59.4 | NC_003324.1 | + | 54492 | 0.71 | 0.2566 |
Target: 5'- --gGC-CCGGCAGCGGUCccgGGGAGUUg -3' miRNA: 3'- cuaUGcGGCCGUCGCCAG---CUCUCGGa -5' |
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12391 | 5' | -59.4 | NC_003324.1 | + | 32470 | 0.7 | 0.290451 |
Target: 5'- --cGCGCCGa-GGUGGUgCGAGAGCCc -3' miRNA: 3'- cuaUGCGGCcgUCGCCA-GCUCUCGGa -5' |
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12391 | 5' | -59.4 | NC_003324.1 | + | 21383 | 0.68 | 0.368376 |
Target: 5'- gGAUAuCGCCGGCgaGGCGG-CGAacgcGGCCg -3' miRNA: 3'- -CUAU-GCGGCCG--UCGCCaGCUc---UCGGa -5' |
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12391 | 5' | -59.4 | NC_003324.1 | + | 40326 | 0.67 | 0.421564 |
Target: 5'- --cACGCaaGGCGGCaGUCGAgcggGAGCCa -3' miRNA: 3'- cuaUGCGg-CCGUCGcCAGCU----CUCGGa -5' |
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12391 | 5' | -59.4 | NC_003324.1 | + | 488 | 0.67 | 0.437455 |
Target: 5'- gGAUGCGCCagcccaauaggcauGGCuuGGCGGUgGuuAGCCg -3' miRNA: 3'- -CUAUGCGG--------------CCG--UCGCCAgCucUCGGa -5' |
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12391 | 5' | -59.4 | NC_003324.1 | + | 31319 | 0.66 | 0.469252 |
Target: 5'- aGUGCgGCCGGCGGCGcuggcgcuggCGGGGGCa- -3' miRNA: 3'- cUAUG-CGGCCGUCGCca--------GCUCUCGga -5' |
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12391 | 5' | -59.4 | NC_003324.1 | + | 20244 | 0.66 | 0.47912 |
Target: 5'- --gGCuGCCGGCAGCGuUCuGGaucGAGCCUc -3' miRNA: 3'- cuaUG-CGGCCGUCGCcAG-CU---CUCGGA- -5' |
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12391 | 5' | -59.4 | NC_003324.1 | + | 14517 | 0.66 | 0.499153 |
Target: 5'- --gACGCCGGCAugGGcCcAGAGCCg -3' miRNA: 3'- cuaUGCGGCCGUcgCCaGcUCUCGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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