Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12394 | 5' | -56.2 | NC_003324.1 | + | 53493 | 0.71 | 0.361013 |
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Target: 5'- cCGAGGCCgaaUGCCGGcgAUgCCCUGa- -3' miRNA: 3'- -GCUCCGGaa-AUGGCCa-UAgGGGGCau -5' |
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| 12394 | 5' | -56.2 | NC_003324.1 | + | 50030 | 0.7 | 0.418228 |
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Target: 5'- uGGGGCCgcugaucggcuCCGGUuUCCgCCCGUAu -3' miRNA: 3'- gCUCCGGaaau-------GGCCAuAGG-GGGCAU- -5' |
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| 12394 | 5' | -56.2 | NC_003324.1 | + | 4588 | 0.66 | 0.653604 |
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Target: 5'- uCGAGGCCUUagUAgUCGcGUcgCCgCCGUAg -3' miRNA: 3'- -GCUCCGGAA--AU-GGC-CAuaGGgGGCAU- -5' |
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| 12394 | 5' | -56.2 | NC_003324.1 | + | 19085 | 0.66 | 0.653604 |
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Target: 5'- uCGAGGCCa--GCCGcGUGcagCCgCCGUAu -3' miRNA: 3'- -GCUCCGGaaaUGGC-CAUa--GGgGGCAU- -5' |
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| 12394 | 5' | -56.2 | NC_003324.1 | + | 29645 | 0.74 | 0.26915 |
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Target: 5'- aCGAGGCCUUgagGCCGcuUGagCCCCGUGc -3' miRNA: 3'- -GCUCCGGAAa--UGGCc-AUagGGGGCAU- -5' |
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| 12394 | 5' | -56.2 | NC_003324.1 | + | 46062 | 1.07 | 0.00121 |
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Target: 5'- uCGAGGCCUUUACCGGUAUCCCCCGUAa -3' miRNA: 3'- -GCUCCGGAAAUGGCCAUAGGGGGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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