Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12399 | 5' | -54.1 | NC_003324.1 | + | 11149 | 0.66 | 0.72781 |
Target: 5'- --uUCGCC---GGUCUGG-CGGCCg- -3' miRNA: 3'- cauAGCGGuauCCAGACCaGUCGGag -5' |
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12399 | 5' | -54.1 | NC_003324.1 | + | 14345 | 0.71 | 0.479657 |
Target: 5'- cUAUCGgCuuGUAGGUCUuuucguagcggucgcGGUCAGCCUUc -3' miRNA: 3'- cAUAGCgG--UAUCCAGA---------------CCAGUCGGAG- -5' |
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12399 | 5' | -54.1 | NC_003324.1 | + | 20829 | 0.67 | 0.704931 |
Target: 5'- -cAUCGCCGUuccggucGGGUCcauGUCGGCCg- -3' miRNA: 3'- caUAGCGGUA-------UCCAGac-CAGUCGGag -5' |
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12399 | 5' | -54.1 | NC_003324.1 | + | 25526 | 0.69 | 0.550173 |
Target: 5'- --cUCGCCAagUGGGUCggcugGGgCGGCCUg -3' miRNA: 3'- cauAGCGGU--AUCCAGa----CCaGUCGGAg -5' |
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12399 | 5' | -54.1 | NC_003324.1 | + | 32107 | 0.66 | 0.72781 |
Target: 5'- -cGUCGCCGgugAGGcCgagugccuUGGUCuccGCCUCg -3' miRNA: 3'- caUAGCGGUa--UCCaG--------ACCAGu--CGGAG- -5' |
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12399 | 5' | -54.1 | NC_003324.1 | + | 35353 | 0.66 | 0.738558 |
Target: 5'- -gGUUGCUGUuGuGcCUGGUCGGCCUg -3' miRNA: 3'- caUAGCGGUAuC-CaGACCAGUCGGAg -5' |
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12399 | 5' | -54.1 | NC_003324.1 | + | 38576 | 0.78 | 0.178394 |
Target: 5'- aUAUCGCCAUcuGGUUUcaccaggcGGUCGGCCUCg -3' miRNA: 3'- cAUAGCGGUAu-CCAGA--------CCAGUCGGAG- -5' |
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12399 | 5' | -54.1 | NC_003324.1 | + | 45088 | 0.68 | 0.650467 |
Target: 5'- --cUCGCCGU---UCUGGUgCAGCUUCa -3' miRNA: 3'- cauAGCGGUAuccAGACCA-GUCGGAG- -5' |
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12399 | 5' | -54.1 | NC_003324.1 | + | 45943 | 0.67 | 0.716963 |
Target: 5'- ---aCGCCGcAGG-CUGGaaAGCCUCc -3' miRNA: 3'- cauaGCGGUaUCCaGACCagUCGGAG- -5' |
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12399 | 5' | -54.1 | NC_003324.1 | + | 49676 | 1.1 | 0.000975 |
Target: 5'- gGUAUCGCCAUAGGUCUGGUCAGCCUCa -3' miRNA: 3'- -CAUAGCGGUAUCCAGACCAGUCGGAG- -5' |
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12399 | 5' | -54.1 | NC_003324.1 | + | 57243 | 0.7 | 0.53925 |
Target: 5'- --uUCGCCAaGGGUCgcaagcGGcCAGCCUg -3' miRNA: 3'- cauAGCGGUaUCCAGa-----CCaGUCGGAg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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