Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 124 | 3' | -53.4 | AC_000006.1 | + | 8073 | 0.67 | 0.672516 |
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Target: 5'- -----gGCGCGGCGGGGGcggaGAGCg -3' miRNA: 3'- gcacgaCGCGCUGCUCCUaaagCUCG- -5' |
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| 124 | 3' | -53.4 | AC_000006.1 | + | 23262 | 0.67 | 0.661133 |
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Target: 5'- gGUGCgguugGCGCGGC-AGGAga-CGGGg -3' miRNA: 3'- gCACGa----CGCGCUGcUCCUaaaGCUCg -5' |
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| 124 | 3' | -53.4 | AC_000006.1 | + | 10864 | 0.67 | 0.626855 |
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Target: 5'- ----aUGCGCGACuGccGGUUUCGGGCg -3' miRNA: 3'- gcacgACGCGCUG-CucCUAAAGCUCG- -5' |
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| 124 | 3' | -53.4 | AC_000006.1 | + | 23118 | 0.68 | 0.604009 |
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Target: 5'- --gGCacGCGCGGCGAGaaGAggUUGAGCu -3' miRNA: 3'- gcaCGa-CGCGCUGCUC--CUaaAGCUCG- -5' |
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| 124 | 3' | -53.4 | AC_000006.1 | + | 19787 | 0.68 | 0.592619 |
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Target: 5'- --aGUUGCGCGA-GGGGAUggagaUGGGCa -3' miRNA: 3'- gcaCGACGCGCUgCUCCUAaa---GCUCG- -5' |
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| 124 | 3' | -53.4 | AC_000006.1 | + | 8695 | 0.69 | 0.514411 |
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Target: 5'- cCGUGgaGCGCGcgggucgcgGCGAucuccaggaggaGGAgaUCGAGCa -3' miRNA: 3'- -GCACgaCGCGC---------UGCU------------CCUaaAGCUCG- -5' |
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| 124 | 3' | -53.4 | AC_000006.1 | + | 15488 | 0.74 | 0.282573 |
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Target: 5'- cCGUGCgucugGCGCGGCGcAGcaGAgcUCGGGCg -3' miRNA: 3'- -GCACGa----CGCGCUGC-UC--CUaaAGCUCG- -5' |
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| 124 | 3' | -53.4 | AC_000006.1 | + | 10922 | 1.11 | 0.000662 |
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Target: 5'- gCGUGCUGCGCGACGAGGAUUUCGAGCc -3' miRNA: 3'- -GCACGACGCGCUGCUCCUAAAGCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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