Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
124 | 5' | -62.7 | AC_000006.1 | + | 10841 | 0.66 | 0.277658 |
Target: 5'- gCAG-CGGGGAggAGCCCgaggagaUGCGCGacuGCc -3' miRNA: 3'- -GUCuGCCCCUagUCGGG-------GCGCGCg--CG- -5' |
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124 | 5' | -62.7 | AC_000006.1 | + | 5198 | 0.66 | 0.239351 |
Target: 5'- --aACGGGcacGGUCuucGCCCUGCGCGUcgGCg -3' miRNA: 3'- gucUGCCC---CUAGu--CGGGGCGCGCG--CG- -5' |
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124 | 5' | -62.7 | AC_000006.1 | + | 15541 | 0.67 | 0.233313 |
Target: 5'- --uGCGGGGGgugcaguggCGGCgCgGCGCGCGg -3' miRNA: 3'- gucUGCCCCUa--------GUCGgGgCGCGCGCg -5' |
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124 | 5' | -62.7 | AC_000006.1 | + | 16692 | 0.67 | 0.233313 |
Target: 5'- aCGGugGGcagaacGAgcgagCGGCCCCG-GCGCGUu -3' miRNA: 3'- -GUCugCCc-----CUa----GUCGGGGCgCGCGCG- -5' |
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124 | 5' | -62.7 | AC_000006.1 | + | 12751 | 0.67 | 0.221623 |
Target: 5'- aGGAgGGGGccacuccCAGCgCCGCGCuggacaugaccGCGCg -3' miRNA: 3'- gUCUgCCCCua-----GUCGgGGCGCG-----------CGCG- -5' |
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124 | 5' | -62.7 | AC_000006.1 | + | 21470 | 0.68 | 0.199736 |
Target: 5'- gCAGuuGGGaccgguGCUCUGCGCGCGCg -3' miRNA: 3'- -GUCugCCCcuagu-CGGGGCGCGCGCG- -5' |
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124 | 5' | -62.7 | AC_000006.1 | + | 10577 | 0.68 | 0.175046 |
Target: 5'- -cGGCGGGGugaccaAGUCCCGCuCGcCGCg -3' miRNA: 3'- guCUGCCCCuag---UCGGGGCGcGC-GCG- -5' |
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124 | 5' | -62.7 | AC_000006.1 | + | 8335 | 0.69 | 0.157288 |
Target: 5'- gCAG-CGGcGGuucCGGCCCCGCGgGCa- -3' miRNA: 3'- -GUCuGCC-CCua-GUCGGGGCGCgCGcg -5' |
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124 | 5' | -62.7 | AC_000006.1 | + | 8726 | 0.7 | 0.126586 |
Target: 5'- cCAGACGcGGcuguaGAccacguccccgUCGGCgUCGCGCGCGCg -3' miRNA: 3'- -GUCUGC-CC-----CU-----------AGUCGgGGCGCGCGCG- -5' |
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124 | 5' | -62.7 | AC_000006.1 | + | 21683 | 0.72 | 0.101514 |
Target: 5'- gCAGugcACGGGcAUCAGCaucaucCCCGCGCcGCGCu -3' miRNA: 3'- -GUC---UGCCCcUAGUCG------GGGCGCG-CGCG- -5' |
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124 | 5' | -62.7 | AC_000006.1 | + | 10956 | 1.04 | 0.000255 |
Target: 5'- gCAGACGGGGAUCAGCCCCGCGCcGCGCa -3' miRNA: 3'- -GUCUGCCCCUAGUCGGGGCGCG-CGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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