Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12402 | 5' | -55.6 | NC_003324.1 | + | 16222 | 0.66 | 0.723334 |
Target: 5'- gCGACgGGgcagGCCGUGCCgaCGCg---- -3' miRNA: 3'- -GUUGgCCa---CGGCGCGGaaGCGaaacu -5' |
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12402 | 5' | -55.6 | NC_003324.1 | + | 21911 | 0.66 | 0.701906 |
Target: 5'- aAGCUGGaUGCCGCGCUgg-GCUUc-- -3' miRNA: 3'- gUUGGCC-ACGGCGCGGaagCGAAacu -5' |
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12402 | 5' | -55.6 | NC_003324.1 | + | 24079 | 0.66 | 0.701906 |
Target: 5'- -cGCCGGUgcGCUGCGCCacggUgGCgcUGAu -3' miRNA: 3'- guUGGCCA--CGGCGCGGa---AgCGaaACU- -5' |
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12402 | 5' | -55.6 | NC_003324.1 | + | 13073 | 0.66 | 0.691085 |
Target: 5'- gCAGCCGGUaccGCCGCcGCCgacCGCc---- -3' miRNA: 3'- -GUUGGCCA---CGGCG-CGGaa-GCGaaacu -5' |
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12402 | 5' | -55.6 | NC_003324.1 | + | 7223 | 0.66 | 0.691085 |
Target: 5'- cCGACgUGGUGCCGauaCCUUCGCa---- -3' miRNA: 3'- -GUUG-GCCACGGCgc-GGAAGCGaaacu -5' |
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12402 | 5' | -55.6 | NC_003324.1 | + | 13706 | 0.67 | 0.647342 |
Target: 5'- gGAUCGGaUGCCgaucgccugcGCGUCUUCGCg--GAa -3' miRNA: 3'- gUUGGCC-ACGG----------CGCGGAAGCGaaaCU- -5' |
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12402 | 5' | -55.6 | NC_003324.1 | + | 55329 | 0.67 | 0.647342 |
Target: 5'- -cGCgCGGUGCCGUccGCCggcacgUCGgaUUGAu -3' miRNA: 3'- guUG-GCCACGGCG--CGGa-----AGCgaAACU- -5' |
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12402 | 5' | -55.6 | NC_003324.1 | + | 28076 | 0.67 | 0.61435 |
Target: 5'- -uGCCGGauggGCuCGUGCCUUUGCgcucgGAg -3' miRNA: 3'- guUGGCCa---CG-GCGCGGAAGCGaaa--CU- -5' |
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12402 | 5' | -55.6 | NC_003324.1 | + | 16144 | 0.68 | 0.592416 |
Target: 5'- gUAGCgGGgGCCGCGCCaggcaUCGCUg--- -3' miRNA: 3'- -GUUGgCCaCGGCGCGGa----AGCGAaacu -5' |
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12402 | 5' | -55.6 | NC_003324.1 | + | 3172 | 0.68 | 0.592416 |
Target: 5'- -cACCGGcaCCGCGCCgaugaaugggaUUGCUUUGAu -3' miRNA: 3'- guUGGCCacGGCGCGGa----------AGCGAAACU- -5' |
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12402 | 5' | -55.6 | NC_003324.1 | + | 54806 | 0.69 | 0.485999 |
Target: 5'- aGACC---GCCGCGCCgUUCGCggUGAg -3' miRNA: 3'- gUUGGccaCGGCGCGG-AAGCGaaACU- -5' |
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12402 | 5' | -55.6 | NC_003324.1 | + | 20590 | 0.7 | 0.445949 |
Target: 5'- gGAuCCGGUGCUgugGCGCCgUCGCcagUGAc -3' miRNA: 3'- gUU-GGCCACGG---CGCGGaAGCGaa-ACU- -5' |
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12402 | 5' | -55.6 | NC_003324.1 | + | 30144 | 0.71 | 0.40781 |
Target: 5'- gCGGCCGGUGUCGCGaCCggcggCGUgaUGGg -3' miRNA: 3'- -GUUGGCCACGGCGC-GGaa---GCGaaACU- -5' |
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12402 | 5' | -55.6 | NC_003324.1 | + | 12161 | 0.71 | 0.38952 |
Target: 5'- aGACCGGUGCC-CGUCUUacCGgUUUGGc -3' miRNA: 3'- gUUGGCCACGGcGCGGAA--GCgAAACU- -5' |
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12402 | 5' | -55.6 | NC_003324.1 | + | 21489 | 0.77 | 0.172663 |
Target: 5'- -uGCCGGaUGCCGCGCCcgUCGCgUUGc -3' miRNA: 3'- guUGGCC-ACGGCGCGGa-AGCGaAACu -5' |
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12402 | 5' | -55.6 | NC_003324.1 | + | 54319 | 1.09 | 0.000981 |
Target: 5'- gCAACCGGUGCCGCGCCUUCGCUUUGAg -3' miRNA: 3'- -GUUGGCCACGGCGCGGAAGCGAAACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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