miRNA display CGI


Results 1 - 3 of 3 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12403 3' -59.9 NC_003324.1 + 2578 0.66 0.494256
Target:  5'- cCAUGGCGacGGaaacuGcAGAGCCCGcaaaUCCGGc -3'
miRNA:   3'- -GUACCGCacCCg----C-UCUCGGGU----AGGCC- -5'
12403 3' -59.9 NC_003324.1 + 32479 0.66 0.471656
Target:  5'- --cGGUaagagcgcgccgagGUGGuGCGAGAGCCCGaCCa- -3'
miRNA:   3'- guaCCG--------------CACC-CGCUCUCGGGUaGGcc -5'
12403 3' -59.9 NC_003324.1 + 55262 1.09 0.000403
Target:  5'- aCAUGGCGUGGGCGAGAGCCCAUCCGGc -3'
miRNA:   3'- -GUACCGCACCCGCUCUCGGGUAGGCC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.