Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12404 | 3' | -51.8 | NC_003324.1 | + | 1774 | 0.66 | 0.9042 |
Target: 5'- cAAGgCGUGGagaucagcgugccagGACGAAC-CGCCGUCa -3' miRNA: 3'- cUUCaGCACCa--------------CUGCUUGcGUGGUAG- -5' |
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12404 | 3' | -51.8 | NC_003324.1 | + | 23547 | 0.66 | 0.86555 |
Target: 5'- -cAG-CGUGGcGGCGGGCGCGCUc-- -3' miRNA: 3'- cuUCaGCACCaCUGCUUGCGUGGuag -5' |
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12404 | 3' | -51.8 | NC_003324.1 | + | 13755 | 0.66 | 0.86555 |
Target: 5'- -cAGUCGUGGUGAagcuguCGAACaccacaaCGCCAUg -3' miRNA: 3'- cuUCAGCACCACU------GCUUGc------GUGGUAg -5' |
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12404 | 3' | -51.8 | NC_003324.1 | + | 28659 | 0.67 | 0.857168 |
Target: 5'- --cGUCGUGGUGccauCGGcAUGCACCu-- -3' miRNA: 3'- cuuCAGCACCACu---GCU-UGCGUGGuag -5' |
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12404 | 3' | -51.8 | NC_003324.1 | + | 5550 | 0.67 | 0.83058 |
Target: 5'- uGAG-CGUGG-GGCGGGCGaCGCCGc- -3' miRNA: 3'- cUUCaGCACCaCUGCUUGC-GUGGUag -5' |
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12404 | 3' | -51.8 | NC_003324.1 | + | 8887 | 0.67 | 0.821268 |
Target: 5'- cGAGGUCGcauUGuUGGCGAACuGUacgGCCAUCa -3' miRNA: 3'- -CUUCAGC---ACcACUGCUUG-CG---UGGUAG- -5' |
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12404 | 3' | -51.8 | NC_003324.1 | + | 22474 | 0.67 | 0.821268 |
Target: 5'- cGgcGagGUGGUGACGGAUugggagcuGuCGCCGUCg -3' miRNA: 3'- -CuuCagCACCACUGCUUG--------C-GUGGUAG- -5' |
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12404 | 3' | -51.8 | NC_003324.1 | + | 39153 | 0.68 | 0.80203 |
Target: 5'- cGAcGUUGUcGGUGACGAucgGCACCuUCu -3' miRNA: 3'- -CUuCAGCA-CCACUGCUug-CGUGGuAG- -5' |
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12404 | 3' | -51.8 | NC_003324.1 | + | 42039 | 0.68 | 0.792127 |
Target: 5'- aGAGUgGUGaacgGACGAugccACGUGCCAUCa -3' miRNA: 3'- cUUCAgCACca--CUGCU----UGCGUGGUAG- -5' |
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12404 | 3' | -51.8 | NC_003324.1 | + | 23953 | 0.73 | 0.528259 |
Target: 5'- cGAAGUCGUucaagacGGUGGCGAGUGCAUCuuUCg -3' miRNA: 3'- -CUUCAGCA-------CCACUGCUUGCGUGGu-AG- -5' |
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12404 | 3' | -51.8 | NC_003324.1 | + | 26009 | 0.73 | 0.518531 |
Target: 5'- cGAcuUCGgGGUGACuGAGCGCGCCAa- -3' miRNA: 3'- -CUucAGCaCCACUG-CUUGCGUGGUag -5' |
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12404 | 3' | -51.8 | NC_003324.1 | + | 55822 | 0.74 | 0.480385 |
Target: 5'- ---cUCGUGGauuuugcggcagcagUGACGGACGCACCAg- -3' miRNA: 3'- cuucAGCACC---------------ACUGCUUGCGUGGUag -5' |
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12404 | 3' | -51.8 | NC_003324.1 | + | 56703 | 1.11 | 0.001706 |
Target: 5'- cGAAGUCGUGGUGACGAACGCACCAUCa -3' miRNA: 3'- -CUUCAGCACCACUGCUUGCGUGGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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