Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12406 | 3' | -57.1 | NC_003324.1 | + | 54510 | 0.66 | 0.581176 |
Target: 5'- cGGcUAUGACCCaGUCgagGCCcgGCaGCGGUc -3' miRNA: 3'- -UC-AUACUGGG-CAGa--CGGa-CGgCGUCG- -5' |
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12406 | 3' | -57.1 | NC_003324.1 | + | 54153 | 0.67 | 0.517307 |
Target: 5'- --gAUGucgaGCCgGUCgGCCgauuccGCCGCAGCg -3' miRNA: 3'- ucaUAC----UGGgCAGaCGGa-----CGGCGUCG- -5' |
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12406 | 3' | -57.1 | NC_003324.1 | + | 52377 | 0.66 | 0.59201 |
Target: 5'- --cAUGcAUCCGUCUG---GCCGCGGCa -3' miRNA: 3'- ucaUAC-UGGGCAGACggaCGGCGUCG- -5' |
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12406 | 3' | -57.1 | NC_003324.1 | + | 51380 | 0.68 | 0.476313 |
Target: 5'- cGUAUGA-UCGUgacGCCUGCCgGCGGCu -3' miRNA: 3'- uCAUACUgGGCAga-CGGACGG-CGUCG- -5' |
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12406 | 3' | -57.1 | NC_003324.1 | + | 47633 | 0.67 | 0.538331 |
Target: 5'- ----cGGCCCucGUCauccaccaugGCaCUGCCGCAGCu -3' miRNA: 3'- ucauaCUGGG--CAGa---------CG-GACGGCGUCG- -5' |
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12406 | 3' | -57.1 | NC_003324.1 | + | 34985 | 0.67 | 0.538331 |
Target: 5'- uGGUGUccgaGCCugcagCGUUUGCguuugCUGCCGCAGCa -3' miRNA: 3'- -UCAUAc---UGG-----GCAGACG-----GACGGCGUCG- -5' |
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12406 | 3' | -57.1 | NC_003324.1 | + | 32028 | 0.76 | 0.16081 |
Target: 5'- aGGaAUGAgCUGUCUGCCgGCgGCGGCu -3' miRNA: 3'- -UCaUACUgGGCAGACGGaCGgCGUCG- -5' |
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12406 | 3' | -57.1 | NC_003324.1 | + | 30523 | 0.67 | 0.52778 |
Target: 5'- cGUGgcGACCUucgCUGCCgcUGCCGCuGCg -3' miRNA: 3'- uCAUa-CUGGGca-GACGG--ACGGCGuCG- -5' |
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12406 | 3' | -57.1 | NC_003324.1 | + | 30046 | 0.7 | 0.364335 |
Target: 5'- ----gGACCCGgCUGCC-GCCGCuacGCa -3' miRNA: 3'- ucauaCUGGGCaGACGGaCGGCGu--CG- -5' |
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12406 | 3' | -57.1 | NC_003324.1 | + | 26867 | 0.68 | 0.486416 |
Target: 5'- ----cGGCCgCGUCUGCCaaGCCGaAGCu -3' miRNA: 3'- ucauaCUGG-GCAGACGGa-CGGCgUCG- -5' |
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12406 | 3' | -57.1 | NC_003324.1 | + | 25593 | 0.7 | 0.37295 |
Target: 5'- cGGUgAUGACauaGUCUGUCgGCCGCuGCu -3' miRNA: 3'- -UCA-UACUGgg-CAGACGGaCGGCGuCG- -5' |
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12406 | 3' | -57.1 | NC_003324.1 | + | 22599 | 0.68 | 0.486416 |
Target: 5'- ----cGGCCUuuuggCUGCCUGCCGCGuuGCc -3' miRNA: 3'- ucauaCUGGGca---GACGGACGGCGU--CG- -5' |
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12406 | 3' | -57.1 | NC_003324.1 | + | 21076 | 0.66 | 0.602878 |
Target: 5'- ---cUGcCuuGUCgGCC-GCCGCGGCg -3' miRNA: 3'- ucauACuGggCAGaCGGaCGGCGUCG- -5' |
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12406 | 3' | -57.1 | NC_003324.1 | + | 16309 | 0.67 | 0.517307 |
Target: 5'- ---cUGGCCgCGUCggcacgGCCUGCCccgucGCGGUa -3' miRNA: 3'- ucauACUGG-GCAGa-----CGGACGG-----CGUCG- -5' |
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12406 | 3' | -57.1 | NC_003324.1 | + | 12114 | 0.66 | 0.613771 |
Target: 5'- --cGUGAUggaCCGUCcGCCcggUGuuGCAGCa -3' miRNA: 3'- ucaUACUG---GGCAGaCGG---ACggCGUCG- -5' |
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12406 | 3' | -57.1 | NC_003324.1 | + | 10968 | 0.69 | 0.436996 |
Target: 5'- cAGUuaacGGCCCGUCguuuaugucgGCCUGCuCGUAGa -3' miRNA: 3'- -UCAua--CUGGGCAGa---------CGGACG-GCGUCg -5' |
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12406 | 3' | -57.1 | NC_003324.1 | + | 10733 | 0.68 | 0.506918 |
Target: 5'- cGGU-UGACCaugGUCUcGCCaGUCGUAGCg -3' miRNA: 3'- -UCAuACUGGg--CAGA-CGGaCGGCGUCG- -5' |
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12406 | 3' | -57.1 | NC_003324.1 | + | 2623 | 0.66 | 0.613771 |
Target: 5'- ----cGugCCGUCUGCCguccGUCGCcuguucgucgguGGCg -3' miRNA: 3'- ucauaCugGGCAGACGGa---CGGCG------------UCG- -5' |
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12406 | 3' | -57.1 | NC_003324.1 | + | 44 | 1.1 | 0.000507 |
Target: 5'- aAGUAUGACCCGUCUGCCUGCCGCAGCc -3' miRNA: 3'- -UCAUACUGGGCAGACGGACGGCGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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