Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12406 | 5' | -60.2 | NC_003324.1 | + | 35468 | 0.66 | 0.406213 |
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Target: 5'- cAUGCGCgGGCgGcCGAGCaGGCUg-- -3' miRNA: 3'- -UACGCGgCCGgCaGCUCGaCUGGuaa -5' |
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| 12406 | 5' | -60.2 | NC_003324.1 | + | 14649 | 0.67 | 0.362238 |
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Target: 5'- uUGCGCCggcaGGCCG-CGAcccauGUUGACCAc- -3' miRNA: 3'- uACGCGG----CCGGCaGCU-----CGACUGGUaa -5' |
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| 12406 | 5' | -60.2 | NC_003324.1 | + | 56797 | 0.67 | 0.35385 |
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Target: 5'- -cGUGCCGGagggUGUCGAGCUuGCCGc- -3' miRNA: 3'- uaCGCGGCCg---GCAGCUCGAcUGGUaa -5' |
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| 12406 | 5' | -60.2 | NC_003324.1 | + | 20708 | 0.67 | 0.337487 |
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Target: 5'- uUGCGCCGGaccaCGggcCGAGCUGAUUu-- -3' miRNA: 3'- uACGCGGCCg---GCa--GCUCGACUGGuaa -5' |
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| 12406 | 5' | -60.2 | NC_003324.1 | + | 6288 | 0.67 | 0.335081 |
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Target: 5'- cAUGCgGCUGGCCGguauccagcuucUCGAcgaggaucacauccGCUGGCCGUUg -3' miRNA: 3'- -UACG-CGGCCGGC------------AGCU--------------CGACUGGUAA- -5' |
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| 12406 | 5' | -60.2 | NC_003324.1 | + | 19455 | 0.68 | 0.291752 |
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Target: 5'- -cGCGCCGG-CGUCGAuccaucGCUGACgGa- -3' miRNA: 3'- uaCGCGGCCgGCAGCU------CGACUGgUaa -5' |
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| 12406 | 5' | -60.2 | NC_003324.1 | + | 36769 | 0.69 | 0.277623 |
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Target: 5'- -aGCGCUGGCCGguguuuUCGAGCUccGGCuCAUc -3' miRNA: 3'- uaCGCGGCCGGC------AGCUCGA--CUG-GUAa -5' |
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| 12406 | 5' | -60.2 | NC_003324.1 | + | 29997 | 0.7 | 0.220743 |
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Target: 5'- uUGgGCCGcGCCGUCcAGCaUGGCCAg- -3' miRNA: 3'- uACgCGGC-CGGCAGcUCG-ACUGGUaa -5' |
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| 12406 | 5' | -60.2 | NC_003324.1 | + | 21103 | 0.73 | 0.152163 |
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Target: 5'- cUGCGUCGGCuuccaCGUCGGGCUGGCg--- -3' miRNA: 3'- uACGCGGCCG-----GCAGCUCGACUGguaa -5' |
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| 12406 | 5' | -60.2 | NC_003324.1 | + | 79 | 1.02 | 0.000842 |
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Target: 5'- uAUGCGCCGGCCGUCGAGCUGACCAUUu -3' miRNA: 3'- -UACGCGGCCGGCAGCUCGACUGGUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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