Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12408 | 5' | -55.5 | NC_003324.1 | + | 34041 | 0.66 | 0.709756 |
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Target: 5'- uCGGGCGGG-UGCUuaaUCCcgacccaUGGAUGucUGCa -3' miRNA: 3'- -GCCCGCCCgACGA---AGG-------ACUUAU--ACGg -5' |
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| 12408 | 5' | -55.5 | NC_003324.1 | + | 24011 | 0.67 | 0.667384 |
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Target: 5'- gCGGuaGGGCUGCUgcgugcgCUUGGAgaagAUGgCg -3' miRNA: 3'- -GCCcgCCCGACGAa------GGACUUa---UACgG- -5' |
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| 12408 | 5' | -55.5 | NC_003324.1 | + | 13097 | 0.67 | 0.645418 |
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Target: 5'- aGGGCGGGCguugaUGCcgcCCUGcagAUcGCCa -3' miRNA: 3'- gCCCGCCCG-----ACGaa-GGACuuaUA-CGG- -5' |
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| 12408 | 5' | -55.5 | NC_003324.1 | + | 54540 | 0.67 | 0.645418 |
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Target: 5'- uCGGGCGGGCaaGCUUUCcGcucucGCCg -3' miRNA: 3'- -GCCCGCCCGa-CGAAGGaCuuauaCGG- -5' |
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| 12408 | 5' | -55.5 | NC_003324.1 | + | 3622 | 0.67 | 0.622304 |
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Target: 5'- gGGuGUGGGCUGCauagcccUUCUUGGcgGggGCCu -3' miRNA: 3'- gCC-CGCCCGACG-------AAGGACUuaUa-CGG- -5' |
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| 12408 | 5' | -55.5 | NC_003324.1 | + | 48980 | 0.69 | 0.525742 |
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Target: 5'- uGuGGCGGGUgGCggagUCCUGGAUGcggGCg -3' miRNA: 3'- gC-CCGCCCGaCGa---AGGACUUAUa--CGg -5' |
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| 12408 | 5' | -55.5 | NC_003324.1 | + | 56274 | 0.7 | 0.484101 |
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Target: 5'- -aGGCGaGGCUGCcguuUUCCggauUGAAgGUGCCa -3' miRNA: 3'- gcCCGC-CCGACG----AAGG----ACUUaUACGG- -5' |
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| 12408 | 5' | -55.5 | NC_003324.1 | + | 2298 | 1.12 | 0.000602 |
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Target: 5'- gCGGGCGGGCUGCUUCCUGAAUAUGCCu -3' miRNA: 3'- -GCCCGCCCGACGAAGGACUUAUACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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