Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12409 | 5' | -51.3 | NC_003324.1 | + | 2455 | 1.11 | 0.002031 |
Target: 5'- cCCCACCAACUUCAAGUUGGCCUAGCGc -3' miRNA: 3'- -GGGUGGUUGAAGUUCAACCGGAUCGC- -5' |
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12409 | 5' | -51.3 | NC_003324.1 | + | 4764 | 0.74 | 0.489365 |
Target: 5'- -aCGCCAuCUUCAAGggUGGCCUuGGCa -3' miRNA: 3'- ggGUGGUuGAAGUUCa-ACCGGA-UCGc -5' |
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12409 | 5' | -51.3 | NC_003324.1 | + | 19557 | 0.72 | 0.587668 |
Target: 5'- aCUCGCCGACggcggUCAuGUUGGaCCgGGCGa -3' miRNA: 3'- -GGGUGGUUGa----AGUuCAACC-GGaUCGC- -5' |
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12409 | 5' | -51.3 | NC_003324.1 | + | 9561 | 0.72 | 0.587668 |
Target: 5'- gCCACCAuuCUUCAGGUagcgGGCCgauccAGCa -3' miRNA: 3'- gGGUGGUu-GAAGUUCAa---CCGGa----UCGc -5' |
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12409 | 5' | -51.3 | NC_003324.1 | + | 35832 | 0.72 | 0.610158 |
Target: 5'- gCCCAgCAGCUccaUCAGGgauucgaccUGcGCCUGGCGa -3' miRNA: 3'- -GGGUgGUUGA---AGUUCa--------AC-CGGAUCGC- -5' |
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12409 | 5' | -51.3 | NC_003324.1 | + | 45429 | 0.71 | 0.644016 |
Target: 5'- gCCGCCuGCUUCAAGUcgcccccuaaaUGGUUgAGCGa -3' miRNA: 3'- gGGUGGuUGAAGUUCA-----------ACCGGaUCGC- -5' |
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12409 | 5' | -51.3 | NC_003324.1 | + | 57062 | 0.71 | 0.666548 |
Target: 5'- uCCgCAUCAACUaugcUCGGGUUGGgCUGGgGa -3' miRNA: 3'- -GG-GUGGUUGA----AGUUCAACCgGAUCgC- -5' |
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12409 | 5' | -51.3 | NC_003324.1 | + | 26278 | 0.68 | 0.824547 |
Target: 5'- aCCCGCUcuCUUCGAGcUGGCUcacuucGCGg -3' miRNA: 3'- -GGGUGGuuGAAGUUCaACCGGau----CGC- -5' |
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12409 | 5' | -51.3 | NC_003324.1 | + | 52291 | 0.68 | 0.842851 |
Target: 5'- aCCGCCcgaUUCGGGgcGGCC-GGCGu -3' miRNA: 3'- gGGUGGuugAAGUUCaaCCGGaUCGC- -5' |
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12409 | 5' | -51.3 | NC_003324.1 | + | 42764 | 0.67 | 0.868539 |
Target: 5'- cCUCGCCAGCgguugCcGGUccuggUGGCCcGGCGa -3' miRNA: 3'- -GGGUGGUUGaa---GuUCA-----ACCGGaUCGC- -5' |
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12409 | 5' | -51.3 | NC_003324.1 | + | 48365 | 0.66 | 0.891905 |
Target: 5'- cUCCaaGCCGGCUUCGcAGUUGcCCUuGGCGc -3' miRNA: 3'- -GGG--UGGUUGAAGU-UCAACcGGA-UCGC- -5' |
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12409 | 5' | -51.3 | NC_003324.1 | + | 8426 | 0.66 | 0.919165 |
Target: 5'- uUCCGCCGGCUgCAGGUgcaGGCggauuuGCGg -3' miRNA: 3'- -GGGUGGUUGAaGUUCAa--CCGgau---CGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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