Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12412 | 3' | -49.1 | NC_003324.1 | + | 42609 | 0.66 | 0.969403 |
Target: 5'- ---cGAUACUUcgUCUGCuuauagucAUCgcgCGGCGCCg -3' miRNA: 3'- gcuaCUAUGAA--AGACG--------UAG---GUCGCGG- -5' |
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12412 | 3' | -49.1 | NC_003324.1 | + | 7909 | 0.66 | 0.965942 |
Target: 5'- gGAgGAaACUUUCUGUucgCCGGC-CCg -3' miRNA: 3'- gCUaCUaUGAAAGACGua-GGUCGcGG- -5' |
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12412 | 3' | -49.1 | NC_003324.1 | + | 38334 | 0.67 | 0.944494 |
Target: 5'- cCGAccUGAUgGCgaUCgGCAuUCCGGCGCUg -3' miRNA: 3'- -GCU--ACUA-UGaaAGaCGU-AGGUCGCGG- -5' |
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12412 | 3' | -49.1 | NC_003324.1 | + | 28270 | 0.67 | 0.944494 |
Target: 5'- uCGGUGGaagcUACaucgCUugcGCGUUCGGCGCCg -3' miRNA: 3'- -GCUACU----AUGaaa-GA---CGUAGGUCGCGG- -5' |
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12412 | 3' | -49.1 | NC_003324.1 | + | 54338 | 0.67 | 0.939336 |
Target: 5'- gCGcUGAUGCcggUCcaugGCAaCCGGUGCCg -3' miRNA: 3'- -GCuACUAUGaa-AGa---CGUaGGUCGCGG- -5' |
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12412 | 3' | -49.1 | NC_003324.1 | + | 35095 | 0.68 | 0.924523 |
Target: 5'- cCGGUGGcAUcaUCUGCcuuuccgucgucgCCAGCGCCg -3' miRNA: 3'- -GCUACUaUGaaAGACGua-----------GGUCGCGG- -5' |
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12412 | 3' | -49.1 | NC_003324.1 | + | 53431 | 0.68 | 0.915705 |
Target: 5'- aGAUGAuUGCUUgagcgUCUGCGcacggaugaagUCCAuGCGCUc -3' miRNA: 3'- gCUACU-AUGAA-----AGACGU-----------AGGU-CGCGG- -5' |
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12412 | 3' | -49.1 | NC_003324.1 | + | 36808 | 0.68 | 0.902091 |
Target: 5'- gGAUGAUgucACUg---GCuaCCAGCGCCa -3' miRNA: 3'- gCUACUA---UGAaagaCGuaGGUCGCGG- -5' |
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12412 | 3' | -49.1 | NC_003324.1 | + | 53791 | 0.69 | 0.894843 |
Target: 5'- ---cGcUGCUUcaUCcaggcgucgcaUGCGUCCAGCGCCu -3' miRNA: 3'- gcuaCuAUGAA--AG-----------ACGUAGGUCGCGG- -5' |
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12412 | 3' | -49.1 | NC_003324.1 | + | 17198 | 0.7 | 0.845504 |
Target: 5'- gGAcGcgGCUUUCgGCGUUCuugAGCGCCg -3' miRNA: 3'- gCUaCuaUGAAAGaCGUAGG---UCGCGG- -5' |
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12412 | 3' | -49.1 | NC_003324.1 | + | 18427 | 0.7 | 0.836376 |
Target: 5'- aCGAUcAUGCUUggcCUGCcaAUCCucGGCGCCc -3' miRNA: 3'- -GCUAcUAUGAAa--GACG--UAGG--UCGCGG- -5' |
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12412 | 3' | -49.1 | NC_003324.1 | + | 13818 | 0.71 | 0.807633 |
Target: 5'- gGAUGAUGuaUUCccgcGuCAUCCAGUGCCu -3' miRNA: 3'- gCUACUAUgaAAGa---C-GUAGGUCGCGG- -5' |
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12412 | 3' | -49.1 | NC_003324.1 | + | 4558 | 0.71 | 0.787451 |
Target: 5'- aGAgGAUGUgagCcGCAUCCGGCGCCu -3' miRNA: 3'- gCUaCUAUGaaaGaCGUAGGUCGCGG- -5' |
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12412 | 3' | -49.1 | NC_003324.1 | + | 48755 | 0.71 | 0.787451 |
Target: 5'- aGGUGAUGCcgagaUGCGcagCCAGCGUCu -3' miRNA: 3'- gCUACUAUGaaag-ACGUa--GGUCGCGG- -5' |
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12412 | 3' | -49.1 | NC_003324.1 | + | 49521 | 0.71 | 0.77709 |
Target: 5'- aGGUGAUAU--UCUGCcagAUCagccaAGCGCCg -3' miRNA: 3'- gCUACUAUGaaAGACG---UAGg----UCGCGG- -5' |
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12412 | 3' | -49.1 | NC_003324.1 | + | 32906 | 0.71 | 0.77709 |
Target: 5'- gCGAUGAUcgagGCgg-CUGCccgCCAuGCGCCg -3' miRNA: 3'- -GCUACUA----UGaaaGACGua-GGU-CGCGG- -5' |
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12412 | 3' | -49.1 | NC_003324.1 | + | 24102 | 0.71 | 0.766565 |
Target: 5'- gCGcUGAUACccuugucguagUUCUGC-UCCAGCGCg -3' miRNA: 3'- -GCuACUAUGa----------AAGACGuAGGUCGCGg -5' |
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12412 | 3' | -49.1 | NC_003324.1 | + | 36708 | 0.72 | 0.73414 |
Target: 5'- uCGAUGAUGCgga-UGCG-CUGGCGCUg -3' miRNA: 3'- -GCUACUAUGaaagACGUaGGUCGCGG- -5' |
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12412 | 3' | -49.1 | NC_003324.1 | + | 35491 | 0.74 | 0.620745 |
Target: 5'- gCGAUcgccGUACcggCUGCuUCCAGCGCCg -3' miRNA: 3'- -GCUAc---UAUGaaaGACGuAGGUCGCGG- -5' |
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12412 | 3' | -49.1 | NC_003324.1 | + | 23188 | 0.78 | 0.414777 |
Target: 5'- gCGAgcgcGGUACcgcUCUGCAUCguGCGCCa -3' miRNA: 3'- -GCUa---CUAUGaa-AGACGUAGguCGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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