Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12412 | 3' | -49.1 | NC_003324.1 | + | 38334 | 0.67 | 0.944494 |
Target: 5'- cCGAccUGAUgGCgaUCgGCAuUCCGGCGCUg -3' miRNA: 3'- -GCU--ACUA-UGaaAGaCGU-AGGUCGCGG- -5' |
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12412 | 3' | -49.1 | NC_003324.1 | + | 7909 | 0.66 | 0.965942 |
Target: 5'- gGAgGAaACUUUCUGUucgCCGGC-CCg -3' miRNA: 3'- gCUaCUaUGAAAGACGua-GGUCGcGG- -5' |
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12412 | 3' | -49.1 | NC_003324.1 | + | 42609 | 0.66 | 0.969403 |
Target: 5'- ---cGAUACUUcgUCUGCuuauagucAUCgcgCGGCGCCg -3' miRNA: 3'- gcuaCUAUGAA--AGACG--------UAG---GUCGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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