Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12413 | 3' | -57.1 | NC_003324.1 | + | 34594 | 0.73 | 0.230756 |
Target: 5'- aUUCCGCUGGcGGCAAGAagGaCCCGa -3' miRNA: 3'- aAAGGCGGCCaCCGUUCUa-CaGGGCg -5' |
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12413 | 3' | -57.1 | NC_003324.1 | + | 27810 | 0.72 | 0.297505 |
Target: 5'- cUUCgCGCCGaaGUGGUucGGUG-CCCGCg -3' miRNA: 3'- aAAG-GCGGC--CACCGuuCUACaGGGCG- -5' |
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12413 | 3' | -57.1 | NC_003324.1 | + | 33462 | 0.71 | 0.328125 |
Target: 5'- -cUCCGCCaGUGGCcGGA-GUUgCGCg -3' miRNA: 3'- aaAGGCGGcCACCGuUCUaCAGgGCG- -5' |
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12413 | 3' | -57.1 | NC_003324.1 | + | 2599 | 0.67 | 0.513481 |
Target: 5'- -gUUCGUCGGUGGCGucacGGcuuacuaucUGUCgCCGCu -3' miRNA: 3'- aaAGGCGGCCACCGUu---CU---------ACAG-GGCG- -5' |
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12413 | 3' | -57.1 | NC_003324.1 | + | 43173 | 0.67 | 0.519746 |
Target: 5'- --cCCGCUGGcggaUGGCGAGAcgacauucucggcGUCCUGCc -3' miRNA: 3'- aaaGGCGGCC----ACCGUUCUa------------CAGGGCG- -5' |
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12413 | 3' | -57.1 | NC_003324.1 | + | 24777 | 0.67 | 0.528145 |
Target: 5'- -gUCgGCgaguggaagggcaaGGaUGGCAAGGcUGUCCCGCu -3' miRNA: 3'- aaAGgCGg-------------CC-ACCGUUCU-ACAGGGCG- -5' |
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12413 | 3' | -57.1 | NC_003324.1 | + | 18523 | 0.66 | 0.620826 |
Target: 5'- --cCCGaCCGGcGGCAAG--GUCgCGCa -3' miRNA: 3'- aaaGGC-GGCCaCCGUUCuaCAGgGCG- -5' |
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12413 | 3' | -57.1 | NC_003324.1 | + | 51296 | 0.66 | 0.620826 |
Target: 5'- -aUCgCGCCGGaguaaaGCAuggGGAUGUCgCGCa -3' miRNA: 3'- aaAG-GCGGCCac----CGU---UCUACAGgGCG- -5' |
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12413 | 3' | -57.1 | NC_003324.1 | + | 20785 | 0.66 | 0.588134 |
Target: 5'- --aCCGCCGGaacguUGGCGAGgcGgcggaCCGUg -3' miRNA: 3'- aaaGGCGGCC-----ACCGUUCuaCag---GGCG- -5' |
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12413 | 3' | -57.1 | NC_003324.1 | + | 50285 | 0.67 | 0.570817 |
Target: 5'- --gCCGCCGGUcgacGGCAAGAaggcguucaccauaGUCCCu- -3' miRNA: 3'- aaaGGCGGCCA----CCGUUCUa-------------CAGGGcg -5' |
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12413 | 3' | -57.1 | NC_003324.1 | + | 29940 | 0.67 | 0.566506 |
Target: 5'- --aCgGuCCGGacgGGCGAGGggcGUCCUGCg -3' miRNA: 3'- aaaGgC-GGCCa--CCGUUCUa--CAGGGCG- -5' |
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12413 | 3' | -57.1 | NC_003324.1 | + | 43104 | 0.67 | 0.540835 |
Target: 5'- -aUCCGCCaGcgGGCAuaaugccaaaGUCCCGCa -3' miRNA: 3'- aaAGGCGGcCa-CCGUucua------CAGGGCG- -5' |
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12413 | 3' | -57.1 | NC_003324.1 | + | 54445 | 0.68 | 0.472571 |
Target: 5'- -gUCauaGCCGGcGGCGAGAgcggaaagcuUG-CCCGCc -3' miRNA: 3'- aaAGg--CGGCCaCCGUUCU----------ACaGGGCG- -5' |
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12413 | 3' | -57.1 | NC_003324.1 | + | 36662 | 0.73 | 0.249343 |
Target: 5'- --gCCGCCGGagauGCGAGAUGUCuuGUc -3' miRNA: 3'- aaaGGCGGCCac--CGUUCUACAGggCG- -5' |
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12413 | 3' | -57.1 | NC_003324.1 | + | 27276 | 0.76 | 0.150479 |
Target: 5'- cUUCCGCguUGGUGGCGcGGAUuUCCCGCc -3' miRNA: 3'- aAAGGCG--GCCACCGU-UCUAcAGGGCG- -5' |
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12413 | 3' | -57.1 | NC_003324.1 | + | 4283 | 1.1 | 0.000559 |
Target: 5'- gUUUCCGCCGGUGGCAAGAUGUCCCGCa -3' miRNA: 3'- -AAAGGCGGCCACCGUUCUACAGGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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