Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12415 | 5' | -54.3 | NC_003324.1 | + | 53056 | 0.71 | 0.477932 |
Target: 5'- uGGCGGcaGGuCGCGCU-CGUCUcggcGCGUCAc -3' miRNA: 3'- -CCGCU--UC-GCGUGAuGCAGA----CGCAGUa -5' |
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12415 | 5' | -54.3 | NC_003324.1 | + | 53999 | 0.69 | 0.572837 |
Target: 5'- cGGCGAAGCGCACUugccGCGcugaugaUCuUGCGgCGg -3' miRNA: 3'- -CCGCUUCGCGUGA----UGC-------AG-ACGCaGUa -5' |
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12415 | 5' | -54.3 | NC_003324.1 | + | 13194 | 0.67 | 0.6924 |
Target: 5'- cGGCGAAGaUGCuaaccgucagcgagGCUGCGUCggcgGCGgUCGg -3' miRNA: 3'- -CCGCUUC-GCG--------------UGAUGCAGa---CGC-AGUa -5' |
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12415 | 5' | -54.3 | NC_003324.1 | + | 26891 | 0.67 | 0.695686 |
Target: 5'- cGGCGccaaagccagccAAGCGCACggccGCGUCUGCc---- -3' miRNA: 3'- -CCGC------------UUCGCGUGa---UGCAGACGcagua -5' |
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12415 | 5' | -54.3 | NC_003324.1 | + | 55537 | 0.67 | 0.705509 |
Target: 5'- aGCGucGAGCGCcaaaucaGCgUGCGUCaUGCGUCGg -3' miRNA: 3'- cCGC--UUCGCG-------UG-AUGCAG-ACGCAGUa -5' |
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12415 | 5' | -54.3 | NC_003324.1 | + | 43373 | 0.66 | 0.780226 |
Target: 5'- --aGAAGCGC-CUGCGag-GCGUCGg -3' miRNA: 3'- ccgCUUCGCGuGAUGCagaCGCAGUa -5' |
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12415 | 5' | -54.3 | NC_003324.1 | + | 28215 | 0.66 | 0.759797 |
Target: 5'- cGGCGAAGuCGCGgCUuCGUgagCcGCGUCAUc -3' miRNA: 3'- -CCGCUUC-GCGU-GAuGCA---GaCGCAGUA- -5' |
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12415 | 5' | -54.3 | NC_003324.1 | + | 29951 | 0.66 | 0.770084 |
Target: 5'- gGGCGAGGgGCGucCUGCGUagcgGCGgCAg -3' miRNA: 3'- -CCGCUUCgCGU--GAUGCAga--CGCaGUa -5' |
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12415 | 5' | -54.3 | NC_003324.1 | + | 8353 | 0.66 | 0.770084 |
Target: 5'- cGGCGggGa--GCUGCGUCUGU--CAUg -3' miRNA: 3'- -CCGCuuCgcgUGAUGCAGACGcaGUA- -5' |
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12415 | 5' | -54.3 | NC_003324.1 | + | 4645 | 1.09 | 0.001245 |
Target: 5'- cGGCGAAGCGCACUACGUCUGCGUCAUg -3' miRNA: 3'- -CCGCUUCGCGUGAUGCAGACGCAGUA- -5' |
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12415 | 5' | -54.3 | NC_003324.1 | + | 23186 | 0.67 | 0.728182 |
Target: 5'- cGGCG-AGCGCGgUAcCGcUCUGCaUCGUg -3' miRNA: 3'- -CCGCuUCGCGUgAU-GC-AGACGcAGUA- -5' |
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12415 | 5' | -54.3 | NC_003324.1 | + | 34462 | 0.67 | 0.717433 |
Target: 5'- cGGCGGGauuuCGCGCU---UCUGCGUCGUc -3' miRNA: 3'- -CCGCUUc---GCGUGAugcAGACGCAGUA- -5' |
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12415 | 5' | -54.3 | NC_003324.1 | + | 39380 | 0.68 | 0.651531 |
Target: 5'- cGGCGAAGCaGC-UUGCG-CUGCGgcUCGUu -3' miRNA: 3'- -CCGCUUCG-CGuGAUGCaGACGC--AGUA- -5' |
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12415 | 5' | -54.3 | NC_003324.1 | + | 21092 | 0.69 | 0.573936 |
Target: 5'- cGCGgcGCGUGCUGCGUCggcuuccaCGUCGg -3' miRNA: 3'- cCGCuuCGCGUGAUGCAGac------GCAGUa -5' |
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12415 | 5' | -54.3 | NC_003324.1 | + | 27858 | 0.69 | 0.552068 |
Target: 5'- aGGCGgcGCGCGCUuCGUCcucgcugGCGUa-- -3' miRNA: 3'- -CCGCuuCGCGUGAuGCAGa------CGCAgua -5' |
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12415 | 5' | -54.3 | NC_003324.1 | + | 18350 | 0.72 | 0.418503 |
Target: 5'- uGGC-AGGCcaaGCAUgauCGUCUGCGUCAUg -3' miRNA: 3'- -CCGcUUCG---CGUGau-GCAGACGCAGUA- -5' |
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12415 | 5' | -54.3 | NC_003324.1 | + | 26182 | 0.79 | 0.157935 |
Target: 5'- cGGCGGucucgcuGCgGgGCUGCGUCUGCGUCGUc -3' miRNA: 3'- -CCGCUu------CG-CgUGAUGCAGACGCAGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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