miRNA: 3'- -CCGU------UGUUGcaACGC-CGGCCa--------GCUCG- -5'
miRNA: 3'- cCGUUGuu-GCAACGC--CGGCCaGC--UCG- -5'
miRNA: 3'- -CCGUUGUUGCaa--CG--------------CCGGCCAgCUCg -5'
miRNA: 3'- -CCGUUgUUGCaa-CGCCG-GC------CAGCUCG- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12416 | 3' | -54.6 | NC_003324.1 | + | 48454 | 0.67 | 0.728906 |
|
Target: 5'- aGGCGccaaggGCAAC--UGCGaGCCGGcuuggagugCGAGCa -3' miRNA: 3'- -CCGU------UGUUGcaACGC-CGGCCa--------GCUCG- -5' |
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| 12416 | 3' | -54.6 | NC_003324.1 | + | 53291 | 0.67 | 0.728906 |
|
Target: 5'- cGCAGCuuuCGUUGUGaaGCCGGcCGccAGCu -3' miRNA: 3'- cCGUUGuu-GCAACGC--CGGCCaGC--UCG- -5' |
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| 12416 | 3' | -54.6 | NC_003324.1 | + | 40496 | 0.67 | 0.725748 |
|
Target: 5'- aGGCGACGACGaagcGCcagacgaaggcgaaGGCCGGU-GAGg -3' miRNA: 3'- -CCGUUGUUGCaa--CG--------------CCGGCCAgCUCg -5' |
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| 12416 | 3' | -54.6 | NC_003324.1 | + | 57378 | 0.66 | 0.799528 |
|
Target: 5'- cGGCGGaGGCGaggGCGGCaCGaccggcGUUGAGCa -3' miRNA: 3'- -CCGUUgUUGCaa-CGCCG-GC------CAGCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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