miRNA: 3'- -CCGuu--GUUGCaa-CGCCGGCCaGCuCG- -5'
miRNA: 3'- -CCGUUGUUGcaaCGCCGGc----CAGCu-CG- -5'
miRNA: 3'- cCGUUGUUgcaa-CGCCGGCC--AGCuCG- -5'
miRNA: 3'- -CCGUUGUUGCAACGCCGGCCAGCUCG- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12416 | 3' | -54.6 | NC_003324.1 | + | 14578 | 0.68 | 0.686206 |
|
Target: 5'- cGGCcugcCGGCGcaaGCGGCCGG-CG-GCg -3' miRNA: 3'- -CCGuu--GUUGCaa-CGCCGGCCaGCuCG- -5' |
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| 12416 | 3' | -54.6 | NC_003324.1 | + | 35036 | 0.68 | 0.686206 |
|
Target: 5'- cGGCGGCAGCa--GCGGCCugaucGUCGucGCu -3' miRNA: 3'- -CCGUUGUUGcaaCGCCGGc----CAGCu-CG- -5' |
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| 12416 | 3' | -54.6 | NC_003324.1 | + | 14797 | 0.68 | 0.664505 |
|
Target: 5'- cGCGGCGAagaacGCGGCCGGcaUCG-GCa -3' miRNA: 3'- cCGUUGUUgcaa-CGCCGGCC--AGCuCG- -5' |
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| 12416 | 3' | -54.6 | NC_003324.1 | + | 4852 | 1.13 | 0.000688 |
|
Target: 5'- cGGCAACAACGUUGCGGCCGGUCGAGCc -3' miRNA: 3'- -CCGUUGUUGCAACGCCGGCCAGCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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