Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12419 | 5' | -54.5 | NC_003324.1 | + | 5760 | 1.08 | 0.001612 |
Target: 5'- uCGAGCGGGACCAUUACGGACGCUUCGu -3' miRNA: 3'- -GCUCGCCCUGGUAAUGCCUGCGAAGC- -5' |
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12419 | 5' | -54.5 | NC_003324.1 | + | 20583 | 0.72 | 0.456427 |
Target: 5'- gCGAGCGGGAuCCggUGCuGugGCgccgUCGc -3' miRNA: 3'- -GCUCGCCCU-GGuaAUGcCugCGa---AGC- -5' |
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12419 | 5' | -54.5 | NC_003324.1 | + | 3537 | 0.72 | 0.466315 |
Target: 5'- gGAGCGGaACCGgcugcaaACGG-CGCUUCGg -3' miRNA: 3'- gCUCGCCcUGGUaa-----UGCCuGCGAAGC- -5' |
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12419 | 5' | -54.5 | NC_003324.1 | + | 7861 | 0.71 | 0.490485 |
Target: 5'- gGGGCGGcaguGACCGUUACccauuucggccuaugGGACGCaUCGa -3' miRNA: 3'- gCUCGCC----CUGGUAAUG---------------CCUGCGaAGC- -5' |
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12419 | 5' | -54.5 | NC_003324.1 | + | 16442 | 0.71 | 0.506918 |
Target: 5'- aGAGCGGGGCUGUUGuCGGGCuUggCGg -3' miRNA: 3'- gCUCGCCCUGGUAAU-GCCUGcGaaGC- -5' |
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12419 | 5' | -54.5 | NC_003324.1 | + | 49733 | 0.7 | 0.548952 |
Target: 5'- -aGGCGGGAUgGUUcaGCGGGCGCa--- -3' miRNA: 3'- gcUCGCCCUGgUAA--UGCCUGCGaagc -5' |
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12419 | 5' | -54.5 | NC_003324.1 | + | 702 | 0.66 | 0.779377 |
Target: 5'- --uGUGGGuACCAUUGCGGGCaccgagcaaaaaaCUUCGg -3' miRNA: 3'- gcuCGCCC-UGGUAAUGCCUGc------------GAAGC- -5' |
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12419 | 5' | -54.5 | NC_003324.1 | + | 11465 | 0.66 | 0.783322 |
Target: 5'- aCGcGCaGGGGCaCAUUGCGGucaagaGCGCcgUCGa -3' miRNA: 3'- -GCuCG-CCCUG-GUAAUGCC------UGCGa-AGC- -5' |
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12419 | 5' | -54.5 | NC_003324.1 | + | 28483 | 0.66 | 0.808333 |
Target: 5'- aCGAGCGuGGccuuACCAUccuugauggcaacGCGGGCGCccUUCGg -3' miRNA: 3'- -GCUCGC-CC----UGGUAa------------UGCCUGCG--AAGC- -5' |
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12419 | 5' | -54.5 | NC_003324.1 | + | 30273 | 0.68 | 0.657405 |
Target: 5'- --cGCGGaGCCGUUGCagaGGGCGuCUUCGa -3' miRNA: 3'- gcuCGCCcUGGUAAUG---CCUGC-GAAGC- -5' |
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12419 | 5' | -54.5 | NC_003324.1 | + | 40311 | 0.7 | 0.567154 |
Target: 5'- uCGAGCGGGAgCCAgcaccgaagcCGGugaaguucgagaaGCGCUUCGu -3' miRNA: 3'- -GCUCGCCCU-GGUaau-------GCC-------------UGCGAAGC- -5' |
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12419 | 5' | -54.5 | NC_003324.1 | + | 57032 | 0.71 | 0.517307 |
Target: 5'- gGAGCGGcGGCgAgggcGCGGAUGcCUUCGa -3' miRNA: 3'- gCUCGCC-CUGgUaa--UGCCUGC-GAAGC- -5' |
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12419 | 5' | -54.5 | NC_003324.1 | + | 37019 | 0.75 | 0.315588 |
Target: 5'- cCGAGCuGGACCucgacgACGGACGCUa-- -3' miRNA: 3'- -GCUCGcCCUGGuaa---UGCCUGCGAagc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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