Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12423 | 3' | -58.5 | NC_003324.1 | + | 49793 | 0.68 | 0.43007 |
Target: 5'- cGCCUGCagggcAGCGGGAUAA-GUgCCGUc -3' miRNA: 3'- aCGGACGc----UCGCCCUGUUgCA-GGCAu -5' |
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12423 | 3' | -58.5 | NC_003324.1 | + | 18955 | 0.68 | 0.411462 |
Target: 5'- aGCCgUGCGugaucuGCGGGACGACuUCCc-- -3' miRNA: 3'- aCGG-ACGCu-----CGCCCUGUUGcAGGcau -5' |
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12423 | 3' | -58.5 | NC_003324.1 | + | 25260 | 0.69 | 0.375783 |
Target: 5'- aUGCCUgGUGAGCuGcGcGACGGCGcCCGUAu -3' miRNA: 3'- -ACGGA-CGCUCG-C-C-CUGUUGCaGGCAU- -5' |
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12423 | 3' | -58.5 | NC_003324.1 | + | 8261 | 1.07 | 0.000686 |
Target: 5'- uUGCCUGCGAGCGGGACAACGUCCGUAg -3' miRNA: 3'- -ACGGACGCUCGCCCUGUUGCAGGCAU- -5' |
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12423 | 3' | -58.5 | NC_003324.1 | + | 35470 | 0.73 | 0.196921 |
Target: 5'- aUGUCgGCGAGCgugGGGACGGCGaucgCCGUAc -3' miRNA: 3'- -ACGGaCGCUCG---CCCUGUUGCa---GGCAU- -5' |
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12423 | 3' | -58.5 | NC_003324.1 | + | 29961 | 0.7 | 0.303458 |
Target: 5'- cGuCCUGCGuAGCGGcGGCAGCcggGUCCGc- -3' miRNA: 3'- aC-GGACGC-UCGCC-CUGUUG---CAGGCau -5' |
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12423 | 3' | -58.5 | NC_003324.1 | + | 28858 | 0.69 | 0.350431 |
Target: 5'- cGCUUGCGAGCcGGAgcagugagcuCAACGUCCc-- -3' miRNA: 3'- aCGGACGCUCGcCCU----------GUUGCAGGcau -5' |
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12423 | 3' | -58.5 | NC_003324.1 | + | 2839 | 0.69 | 0.367196 |
Target: 5'- cGCCggGCGAGgGGGAgcguCAACGgCCGa- -3' miRNA: 3'- aCGGa-CGCUCgCCCU----GUUGCaGGCau -5' |
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12423 | 3' | -58.5 | NC_003324.1 | + | 7844 | 0.69 | 0.384505 |
Target: 5'- gGCUUGCGcagaauGGCGGGGCGGCaGUgaCCGUu -3' miRNA: 3'- aCGGACGC------UCGCCCUGUUG-CA--GGCAu -5' |
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12423 | 3' | -58.5 | NC_003324.1 | + | 46321 | 0.67 | 0.449165 |
Target: 5'- aGCUcGCGAGCGcGGugGGCucugCCGUGu -3' miRNA: 3'- aCGGaCGCUCGC-CCugUUGca--GGCAU- -5' |
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12423 | 3' | -58.5 | NC_003324.1 | + | 30593 | 0.67 | 0.498836 |
Target: 5'- uUGCC-GCGAGCGaGGAUGcCGcCCGg- -3' miRNA: 3'- -ACGGaCGCUCGC-CCUGUuGCaGGCau -5' |
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12423 | 3' | -58.5 | NC_003324.1 | + | 21760 | 0.66 | 0.519386 |
Target: 5'- gGCuCUGCaGGGCGaGGAacuCGUCCGg- -3' miRNA: 3'- aCG-GACG-CUCGC-CCUguuGCAGGCau -5' |
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12423 | 3' | -58.5 | NC_003324.1 | + | 54298 | 0.66 | 0.540262 |
Target: 5'- gGCCUGCGucuGCGccgucuGC-ACGUCCGUGg -3' miRNA: 3'- aCGGACGCu--CGCcc----UGuUGCAGGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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