Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12424 | 3' | -55.5 | NC_003324.1 | + | 48423 | 0.66 | 0.710829 |
Target: 5'- cGCUUCaCCGccucaucgaagaGCGCCG-CGCCGAGAa- -3' miRNA: 3'- -UGAAGaGGC------------UGUGGCgGCGGUUCUag -5' |
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12424 | 3' | -55.5 | NC_003324.1 | + | 32574 | 0.66 | 0.710829 |
Target: 5'- gACaUCgUCGACACCGUgauCGCCGAGGc- -3' miRNA: 3'- -UGaAGaGGCUGUGGCG---GCGGUUCUag -5' |
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12424 | 3' | -55.5 | NC_003324.1 | + | 43708 | 0.66 | 0.700055 |
Target: 5'- --gUCgUCCGGUACCGCCugcuGCCAaucgGGAUCa -3' miRNA: 3'- ugaAG-AGGCUGUGGCGG----CGGU----UCUAG- -5' |
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12424 | 3' | -55.5 | NC_003324.1 | + | 35538 | 0.66 | 0.678322 |
Target: 5'- cGCUcg-CCGACAUCGCCGaCGuGAUCc -3' miRNA: 3'- -UGAagaGGCUGUGGCGGCgGUuCUAG- -5' |
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12424 | 3' | -55.5 | NC_003324.1 | + | 20259 | 0.66 | 0.677229 |
Target: 5'- cGCcUCUCUGACcgagGCUGCCGgCAgcguucuGGAUCg -3' miRNA: 3'- -UGaAGAGGCUG----UGGCGGCgGU-------UCUAG- -5' |
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12424 | 3' | -55.5 | NC_003324.1 | + | 49648 | 0.67 | 0.667383 |
Target: 5'- aACUUCgaCGACGCUGCCGauauCAAGggCg -3' miRNA: 3'- -UGAAGagGCUGUGGCGGCg---GUUCuaG- -5' |
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12424 | 3' | -55.5 | NC_003324.1 | + | 36732 | 0.67 | 0.656412 |
Target: 5'- cGCaUCUCCGgcgGCACCuUCGCCGGGGa- -3' miRNA: 3'- -UGaAGAGGC---UGUGGcGGCGGUUCUag -5' |
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12424 | 3' | -55.5 | NC_003324.1 | + | 13335 | 0.67 | 0.656412 |
Target: 5'- -gUUCUUCGACGCuUGCgGCCAuGAUg -3' miRNA: 3'- ugAAGAGGCUGUG-GCGgCGGUuCUAg -5' |
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12424 | 3' | -55.5 | NC_003324.1 | + | 45298 | 0.67 | 0.656412 |
Target: 5'- uGCUUCcagUUCGA-GCCGgCGCUggGAUCg -3' miRNA: 3'- -UGAAG---AGGCUgUGGCgGCGGuuCUAG- -5' |
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12424 | 3' | -55.5 | NC_003324.1 | + | 14223 | 0.67 | 0.623404 |
Target: 5'- cGCUUCUCgGAgACCGCaaaCGCCucGcgCg -3' miRNA: 3'- -UGAAGAGgCUgUGGCG---GCGGuuCuaG- -5' |
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12424 | 3' | -55.5 | NC_003324.1 | + | 54412 | 0.67 | 0.612404 |
Target: 5'- aACUcC-CCGGgACCGCUGCCGggccucgacuGGGUCa -3' miRNA: 3'- -UGAaGaGGCUgUGGCGGCGGU----------UCUAG- -5' |
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12424 | 3' | -55.5 | NC_003324.1 | + | 43702 | 0.68 | 0.594843 |
Target: 5'- gUUUCUCCGGCuugcuguCCGagcagucgagcgcgaCCGCCAAGuAUCa -3' miRNA: 3'- uGAAGAGGCUGu------GGC---------------GGCGGUUC-UAG- -5' |
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12424 | 3' | -55.5 | NC_003324.1 | + | 38071 | 0.68 | 0.579541 |
Target: 5'- aACcgCUCCGGCAUgGCCGCCu----- -3' miRNA: 3'- -UGaaGAGGCUGUGgCGGCGGuucuag -5' |
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12424 | 3' | -55.5 | NC_003324.1 | + | 20747 | 0.68 | 0.557837 |
Target: 5'- gGCUUC-CaaGCAUCGCCGCCu-GGUCg -3' miRNA: 3'- -UGAAGaGgcUGUGGCGGCGGuuCUAG- -5' |
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12424 | 3' | -55.5 | NC_003324.1 | + | 31726 | 0.69 | 0.504737 |
Target: 5'- aACUUCUCCGGCG-CGCCGa-AGGAa- -3' miRNA: 3'- -UGAAGAGGCUGUgGCGGCggUUCUag -5' |
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12424 | 3' | -55.5 | NC_003324.1 | + | 31644 | 0.69 | 0.501616 |
Target: 5'- cACUUcCUUCGGCgcgccggagaaguuGCCGCCGuuccauCCGAGAUCg -3' miRNA: 3'- -UGAA-GAGGCUG--------------UGGCGGC------GGUUCUAG- -5' |
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12424 | 3' | -55.5 | NC_003324.1 | + | 45901 | 0.7 | 0.473936 |
Target: 5'- cGCUccUCaUUCGGCGCCGCCGCUucGGAa- -3' miRNA: 3'- -UGA--AG-AGGCUGUGGCGGCGGu-UCUag -5' |
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12424 | 3' | -55.5 | NC_003324.1 | + | 33654 | 0.71 | 0.406036 |
Target: 5'- uGCUUCUCCccuucuacgcucGauacgccuGCGCUGCCGCCAAGGcaUCa -3' miRNA: 3'- -UGAAGAGG------------C--------UGUGGCGGCGGUUCU--AG- -5' |
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12424 | 3' | -55.5 | NC_003324.1 | + | 18883 | 0.71 | 0.394112 |
Target: 5'- cCUUCgCCGcgccauggacccgcGCAUCGuuGCCGAGAUCg -3' miRNA: 3'- uGAAGaGGC--------------UGUGGCggCGGUUCUAG- -5' |
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12424 | 3' | -55.5 | NC_003324.1 | + | 44003 | 0.71 | 0.387786 |
Target: 5'- ---cCUCCucgGCUGCCGCCGGGAUCa -3' miRNA: 3'- ugaaGAGGcugUGGCGGCGGUUCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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