Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12424 | 5' | -55 | NC_003324.1 | + | 33437 | 0.66 | 0.76672 |
Target: 5'- cGGAGCCGgcaaccacGCACCGGGAcgAUGacCAUGu -3' miRNA: 3'- aCCUCGGC--------UGUGGCUCU--UACcaGUGC- -5' |
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12424 | 5' | -55 | NC_003324.1 | + | 9384 | 0.67 | 0.714727 |
Target: 5'- uUGGuGCCGcCACCGAuGAcgaGGUCuCGg -3' miRNA: 3'- -ACCuCGGCuGUGGCU-CUua-CCAGuGC- -5' |
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12424 | 5' | -55 | NC_003324.1 | + | 15205 | 0.67 | 0.714727 |
Target: 5'- gUGGcgccguGUCGACGCCGAGAccgAUGG-CAUc -3' miRNA: 3'- -ACCu-----CGGCUGUGGCUCU---UACCaGUGc -5' |
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12424 | 5' | -55 | NC_003324.1 | + | 30799 | 0.67 | 0.714727 |
Target: 5'- aGGcGGCCGACGCCaAGAAUuGGUgggaCACc -3' miRNA: 3'- aCC-UCGGCUGUGGcUCUUA-CCA----GUGc -5' |
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12424 | 5' | -55 | NC_003324.1 | + | 44422 | 0.68 | 0.627905 |
Target: 5'- gUGcGGGCaccgGGCGCCGAGAcuucGGUCGCu -3' miRNA: 3'- -AC-CUCGg---CUGUGGCUCUua--CCAGUGc -5' |
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12424 | 5' | -55 | NC_003324.1 | + | 27757 | 0.68 | 0.616963 |
Target: 5'- aGGAGaagcuCGGCGCCacgcauGAG-GUGGUCGCGa -3' miRNA: 3'- aCCUCg----GCUGUGG------CUCuUACCAGUGC- -5' |
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12424 | 5' | -55 | NC_003324.1 | + | 18151 | 0.68 | 0.606034 |
Target: 5'- cGGuuucGCCGACACCGgcaAGGucGUGGcUCugGg -3' miRNA: 3'- aCCu---CGGCUGUGGC---UCU--UACC-AGugC- -5' |
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12424 | 5' | -55 | NC_003324.1 | + | 48446 | 0.68 | 0.606034 |
Target: 5'- ---cGCCG-CGCCGAGAagAUGGUgCGCGc -3' miRNA: 3'- accuCGGCuGUGGCUCU--UACCA-GUGC- -5' |
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12424 | 5' | -55 | NC_003324.1 | + | 20835 | 0.68 | 0.604942 |
Target: 5'- aGGAgcgcaugGCCGACAUCGAGAAgcaGG-CGCc -3' miRNA: 3'- aCCU-------CGGCUGUGGCUCUUa--CCaGUGc -5' |
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12424 | 5' | -55 | NC_003324.1 | + | 2851 | 0.69 | 0.562626 |
Target: 5'- gGGAGCgucaACgGCCGAGAggugcgagGUGGUCACGc -3' miRNA: 3'- aCCUCGgc--UG-UGGCUCU--------UACCAGUGC- -5' |
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12424 | 5' | -55 | NC_003324.1 | + | 36976 | 0.71 | 0.45889 |
Target: 5'- gUGG-GUCGACGCCGAcauUGGUUGCGc -3' miRNA: 3'- -ACCuCGGCUGUGGCUcuuACCAGUGC- -5' |
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12424 | 5' | -55 | NC_003324.1 | + | 16149 | 0.73 | 0.352652 |
Target: 5'- gGGGGCCGcgccaggcaucgcugGCACCGAGGcgGG-CACc -3' miRNA: 3'- aCCUCGGC---------------UGUGGCUCUuaCCaGUGc -5' |
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12424 | 5' | -55 | NC_003324.1 | + | 8696 | 1.09 | 0.001297 |
Target: 5'- gUGGAGCCGACACCGAGAAUGGUCACGu -3' miRNA: 3'- -ACCUCGGCUGUGGCUCUUACCAGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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