Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12425 | 5' | -49.5 | NC_003324.1 | + | 13626 | 0.66 | 0.956163 |
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Target: 5'- ------cGCGCAGGCGAUcgGCAUCCg- -3' miRNA: 3'- acauagaUGUGUCCGUUG--UGUAGGgu -5' |
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| 12425 | 5' | -49.5 | NC_003324.1 | + | 32309 | 0.66 | 0.956163 |
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Target: 5'- ---cUCUGCGC-GGCGcCGCAUCuCCGg -3' miRNA: 3'- acauAGAUGUGuCCGUuGUGUAG-GGU- -5' |
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| 12425 | 5' | -49.5 | NC_003324.1 | + | 45672 | 0.66 | 0.956163 |
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Target: 5'- ------aAUACAGGCGcGCACAUCCgCAg -3' miRNA: 3'- acauagaUGUGUCCGU-UGUGUAGG-GU- -5' |
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| 12425 | 5' | -49.5 | NC_003324.1 | + | 53459 | 0.66 | 0.941906 |
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Target: 5'- cGUAUCaACGCcGGCAAgUACA-CCCGc -3' miRNA: 3'- aCAUAGaUGUGuCCGUU-GUGUaGGGU- -5' |
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| 12425 | 5' | -49.5 | NC_003324.1 | + | 25292 | 0.66 | 0.936555 |
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Target: 5'- cGUcaGUCgGCGCAGGCAACu--UCCUu -3' miRNA: 3'- aCA--UAGaUGUGUCCGUUGuguAGGGu -5' |
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| 12425 | 5' | -49.5 | NC_003324.1 | + | 28713 | 0.67 | 0.903109 |
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Target: 5'- --gGUCUGgGCAGGCGccgaaaccugcucuGCAguUCCCGc -3' miRNA: 3'- acaUAGAUgUGUCCGU--------------UGUguAGGGU- -5' |
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| 12425 | 5' | -49.5 | NC_003324.1 | + | 50060 | 0.7 | 0.811218 |
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Target: 5'- cGUAUCU-CGCGGGCggUu--UCCCGa -3' miRNA: 3'- aCAUAGAuGUGUCCGuuGuguAGGGU- -5' |
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| 12425 | 5' | -49.5 | NC_003324.1 | + | 6242 | 0.73 | 0.635024 |
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Target: 5'- -aUGUCgu--CGGGCAGCGCAUUCCAg -3' miRNA: 3'- acAUAGauguGUCCGUUGUGUAGGGU- -5' |
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| 12425 | 5' | -49.5 | NC_003324.1 | + | 9802 | 1.1 | 0.003185 |
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Target: 5'- cUGUAUCUACACAGGCAACACAUCCCAa -3' miRNA: 3'- -ACAUAGAUGUGUCCGUUGUGUAGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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