Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12426 | 5' | -55.5 | NC_003324.1 | + | 32365 | 0.68 | 0.645683 |
Target: 5'- gCCGuUGUCGCcaGCGuguucuCUGGCGAGcguCCc -3' miRNA: 3'- aGGC-ACAGCGa-UGCu-----GACCGCUCu--GG- -5' |
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12426 | 5' | -55.5 | NC_003324.1 | + | 57180 | 0.76 | 0.228984 |
Target: 5'- cCCuUG-CGCUGCGAg-GGCGAGGCCa -3' miRNA: 3'- aGGcACaGCGAUGCUgaCCGCUCUGG- -5' |
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12426 | 5' | -55.5 | NC_003324.1 | + | 28988 | 0.7 | 0.507177 |
Target: 5'- gCCGgaaucgGUCGCaGCGAa-GGaCGAGGCCg -3' miRNA: 3'- aGGCa-----CAGCGaUGCUgaCC-GCUCUGG- -5' |
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12426 | 5' | -55.5 | NC_003324.1 | + | 24157 | 0.69 | 0.591611 |
Target: 5'- aCCGUGgCGCaGCGcACcGGCGAGAa- -3' miRNA: 3'- aGGCACaGCGaUGC-UGaCCGCUCUgg -5' |
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12426 | 5' | -55.5 | NC_003324.1 | + | 30141 | 0.68 | 0.602394 |
Target: 5'- gCCgGUGUCGCgaccgGCGGCgugaugGGCGGcGGCa -3' miRNA: 3'- aGG-CACAGCGa----UGCUGa-----CCGCU-CUGg -5' |
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12426 | 5' | -55.5 | NC_003324.1 | + | 39075 | 0.68 | 0.602394 |
Target: 5'- aCCGgcucacUCGCUGCGGCUGcGCaGGGCa -3' miRNA: 3'- aGGCac----AGCGAUGCUGAC-CGcUCUGg -5' |
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12426 | 5' | -55.5 | NC_003324.1 | + | 41951 | 0.68 | 0.613202 |
Target: 5'- aCUGcUGcUCGCcAUGACcGGCGAGACg -3' miRNA: 3'- aGGC-AC-AGCGaUGCUGaCCGCUCUGg -5' |
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12426 | 5' | -55.5 | NC_003324.1 | + | 3652 | 0.68 | 0.613202 |
Target: 5'- --aGUGUCGCUcuGCuGCaUGGCGGGcGCCa -3' miRNA: 3'- aggCACAGCGA--UGcUG-ACCGCUC-UGG- -5' |
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12426 | 5' | -55.5 | NC_003324.1 | + | 57403 | 0.68 | 0.642436 |
Target: 5'- cCCGUGUCcucacugcuggaggGCU-CGGCggaGGCGAGGgCg -3' miRNA: 3'- aGGCACAG--------------CGAuGCUGa--CCGCUCUgG- -5' |
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12426 | 5' | -55.5 | NC_003324.1 | + | 31313 | 0.67 | 0.688776 |
Target: 5'- gCCGgcggCGCUgGCG-CUGGCGGGGgCa -3' miRNA: 3'- aGGCaca-GCGA-UGCuGACCGCUCUgG- -5' |
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12426 | 5' | -55.5 | NC_003324.1 | + | 30385 | 0.66 | 0.719528 |
Target: 5'- cUCCGgcGUCGCUACaGGCauguucGGCGGcuugggcGACCg -3' miRNA: 3'- -AGGCa-CAGCGAUG-CUGa-----CCGCU-------CUGG- -5' |
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12426 | 5' | -55.5 | NC_003324.1 | + | 10640 | 1.13 | 0.00064 |
Target: 5'- cUCCGUGUCGCUACGACUGGCGAGACCa -3' miRNA: 3'- -AGGCACAGCGAUGCUGACCGCUCUGG- -5' |
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12426 | 5' | -55.5 | NC_003324.1 | + | 38702 | 0.67 | 0.688776 |
Target: 5'- gUCGgcaaaGUCGCUcCGACgGGuCGAGACa -3' miRNA: 3'- aGGCa----CAGCGAuGCUGaCC-GCUCUGg -5' |
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12426 | 5' | -55.5 | NC_003324.1 | + | 26808 | 0.67 | 0.678056 |
Target: 5'- gCCGUG-CGCUugG-CUGGCuuuGGcGCCg -3' miRNA: 3'- aGGCACaGCGAugCuGACCGc--UC-UGG- -5' |
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12426 | 5' | -55.5 | NC_003324.1 | + | 32083 | 0.67 | 0.667294 |
Target: 5'- cUUCGgccUCGCUGCGccucggcucgucGCcGGUGAGGCCg -3' miRNA: 3'- -AGGCac-AGCGAUGC------------UGaCCGCUCUGG- -5' |
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12426 | 5' | -55.5 | NC_003324.1 | + | 32265 | 0.7 | 0.501033 |
Target: 5'- gUCGUGUCGggACGcucgccagagaacacGCUGGCGAcaacGGCCu -3' miRNA: 3'- aGGCACAGCgaUGC---------------UGACCGCU----CUGG- -5' |
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12426 | 5' | -55.5 | NC_003324.1 | + | 28237 | 0.72 | 0.390755 |
Target: 5'- gCCGcGUCaucgacuGCUGCGACUGGUGccGGAUCg -3' miRNA: 3'- aGGCaCAG-------CGAUGCUGACCGC--UCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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