Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12427 | 3' | -63.4 | NC_003324.1 | + | 42971 | 0.66 | 0.336171 |
Target: 5'- cCGCUgggUCGUucuCGGUCgcagauccucgcgGGcCGGCCGGCa- -3' miRNA: 3'- -GCGA---AGCG---GCCAGa------------CC-GCCGGCCGag -5' |
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12427 | 3' | -63.4 | NC_003324.1 | + | 26813 | 0.66 | 0.347091 |
Target: 5'- gCGCUUgGCUGGcUUUGGCG-CC-GCUCc -3' miRNA: 3'- -GCGAAgCGGCC-AGACCGCcGGcCGAG- -5' |
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12427 | 3' | -63.4 | NC_003324.1 | + | 14575 | 0.67 | 0.261689 |
Target: 5'- uCGCggccUGCCGGcgCaaGCGGCCGGCg- -3' miRNA: 3'- -GCGaa--GCGGCCa-GacCGCCGGCCGag -5' |
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12427 | 3' | -63.4 | NC_003324.1 | + | 1022 | 0.67 | 0.261689 |
Target: 5'- aGC-UCGCUGac--GGCGGCgGGCUCa -3' miRNA: 3'- gCGaAGCGGCcagaCCGCCGgCCGAG- -5' |
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12427 | 3' | -63.4 | NC_003324.1 | + | 39446 | 0.67 | 0.261689 |
Target: 5'- uGCUUCGCCGGaUgUGGCa-CCGGaacaUCg -3' miRNA: 3'- gCGAAGCGGCC-AgACCGccGGCCg---AG- -5' |
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12427 | 3' | -63.4 | NC_003324.1 | + | 41867 | 0.69 | 0.193699 |
Target: 5'- aCGUcUCGCCGGUCaUGGCGaGCagcagugUGGUUCc -3' miRNA: 3'- -GCGaAGCGGCCAG-ACCGC-CG-------GCCGAG- -5' |
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12427 | 3' | -63.4 | NC_003324.1 | + | 13169 | 0.69 | 0.220253 |
Target: 5'- gGCUgCGUCGG-C-GGCGGUCGGCg- -3' miRNA: 3'- gCGAaGCGGCCaGaCCGCCGGCCGag -5' |
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12427 | 3' | -63.4 | NC_003324.1 | + | 27867 | 0.69 | 0.214812 |
Target: 5'- gCGCUUCGUCcucgCUGGCGuaGCCGGC-Ca -3' miRNA: 3'- -GCGAAGCGGcca-GACCGC--CGGCCGaG- -5' |
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12427 | 3' | -63.4 | NC_003324.1 | + | 53186 | 0.71 | 0.150082 |
Target: 5'- aGCaugUgGUgGGUgguagCUGGCGGCCGGCUUc -3' miRNA: 3'- gCGa--AgCGgCCA-----GACCGCCGGCCGAG- -5' |
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12427 | 3' | -63.4 | NC_003324.1 | + | 19517 | 0.71 | 0.150082 |
Target: 5'- gGCUUUGCCGGUaaGGacaaGGCCaGGCg- -3' miRNA: 3'- gCGAAGCGGCCAgaCCg---CCGG-CCGag -5' |
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12427 | 3' | -63.4 | NC_003324.1 | + | 32747 | 0.73 | 0.115273 |
Target: 5'- cCGC--CGaCCGGUUUGGUGGCgGGCUUu -3' miRNA: 3'- -GCGaaGC-GGCCAGACCGCCGgCCGAG- -5' |
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12427 | 3' | -63.4 | NC_003324.1 | + | 42757 | 0.76 | 0.061818 |
Target: 5'- aGCggUUGCCGGUcCUGGUGGCcCGGCg- -3' miRNA: 3'- gCGa-AGCGGCCA-GACCGCCG-GCCGag -5' |
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12427 | 3' | -63.4 | NC_003324.1 | + | 23668 | 0.76 | 0.063538 |
Target: 5'- uGCUUCGCCGGUauccacGGCGcGCCgGGCUUc -3' miRNA: 3'- gCGAAGCGGCCAga----CCGC-CGG-CCGAG- -5' |
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12427 | 3' | -63.4 | NC_003324.1 | + | 11145 | 1.09 | 0.000171 |
Target: 5'- cCGCUUCGCCGGUCUGGCGGCCGGCUCg -3' miRNA: 3'- -GCGAAGCGGCCAGACCGCCGGCCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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