miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12427 5' -57.3 NC_003324.1 + 11182 1.06 0.000935
Target:  5'- gAGGUGCGGAUGACCACGACCGGCAAAa -3'
miRNA:   3'- -UCCACGCCUACUGGUGCUGGCCGUUU- -5'
12427 5' -57.3 NC_003324.1 + 18142 0.72 0.287301
Target:  5'- gAGGUGUGGA--ACCGCGAagugaCGGCAc- -3'
miRNA:   3'- -UCCACGCCUacUGGUGCUg----GCCGUuu -5'
12427 5' -57.3 NC_003324.1 + 57385 0.71 0.294554
Target:  5'- gAGGgcucgGCGGAggcgagGGCggCACGACCGGCGu- -3'
miRNA:   3'- -UCCa----CGCCUa-----CUG--GUGCUGGCCGUuu -5'
12427 5' -57.3 NC_003324.1 + 11233 0.7 0.374904
Target:  5'- -uGUGCGaGccGGCCGCcaGACCGGCGAAg -3'
miRNA:   3'- ucCACGC-CuaCUGGUG--CUGGCCGUUU- -5'
12427 5' -57.3 NC_003324.1 + 42644 0.68 0.493137
Target:  5'- cGGGcGCGGAUcugcaacagcaaGGCCAUucgccgggccaccagGACCGGCAAc -3'
miRNA:   3'- -UCCaCGCCUA------------CUGGUG---------------CUGGCCGUUu -5'
12427 5' -57.3 NC_003324.1 + 16142 0.67 0.499303
Target:  5'- cGGUaGCGGG-GGCCGCG-CCaGGCAu- -3'
miRNA:   3'- uCCA-CGCCUaCUGGUGCuGG-CCGUuu -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.