Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12429 | 5' | -52 | NC_003324.1 | + | 34019 | 0.66 | 0.890309 |
Target: 5'- gUCGgGGCCgauGCAGAAGGCGAg---- -3' miRNA: 3'- -GGUgCCGGag-UGUCUUCUGCUagaua -5' |
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12429 | 5' | -52 | NC_003324.1 | + | 7401 | 0.66 | 0.86686 |
Target: 5'- cCCuCGGCg-CGCGGAAcGACGGUCa-- -3' miRNA: 3'- -GGuGCCGgaGUGUCUU-CUGCUAGaua -5' |
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12429 | 5' | -52 | NC_003324.1 | + | 7761 | 0.67 | 0.849941 |
Target: 5'- gCAC-GCCUCGCAGGgaGGcGCGAUCg-- -3' miRNA: 3'- gGUGcCGGAGUGUCU--UC-UGCUAGaua -5' |
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12429 | 5' | -52 | NC_003324.1 | + | 49008 | 0.67 | 0.832069 |
Target: 5'- gCgACGGCCUUgacCAGGucGACGAUCUu- -3' miRNA: 3'- -GgUGCCGGAGu--GUCUu-CUGCUAGAua -5' |
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12429 | 5' | -52 | NC_003324.1 | + | 5075 | 0.67 | 0.813318 |
Target: 5'- aCGCGGCCUgAUAGcuuaagcggcgcGAucGACGAUCUGc -3' miRNA: 3'- gGUGCCGGAgUGUC------------UU--CUGCUAGAUa -5' |
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12429 | 5' | -52 | NC_003324.1 | + | 29931 | 0.68 | 0.803639 |
Target: 5'- gCGCGGCC-CACGGuccGGACGggCg-- -3' miRNA: 3'- gGUGCCGGaGUGUCu--UCUGCuaGaua -5' |
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12429 | 5' | -52 | NC_003324.1 | + | 52454 | 0.68 | 0.792776 |
Target: 5'- gCCGCGGCCagACGGAugcaugcAGGCGAg---- -3' miRNA: 3'- -GGUGCCGGagUGUCU-------UCUGCUagaua -5' |
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12429 | 5' | -52 | NC_003324.1 | + | 45944 | 0.7 | 0.676255 |
Target: 5'- aUCACGGCCUCGCAucAGGCGc----- -3' miRNA: 3'- -GGUGCCGGAGUGUcuUCUGCuagaua -5' |
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12429 | 5' | -52 | NC_003324.1 | + | 22949 | 0.71 | 0.630241 |
Target: 5'- gCACGGUUcauucagUCGCAGAuGGACGAUCUu- -3' miRNA: 3'- gGUGCCGG-------AGUGUCU-UCUGCUAGAua -5' |
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12429 | 5' | -52 | NC_003324.1 | + | 35767 | 0.71 | 0.624616 |
Target: 5'- uCCAUGGCCUCAauaauGAGaucgagcauggcgauGGCGAUCUGg -3' miRNA: 3'- -GGUGCCGGAGUgu---CUU---------------CUGCUAGAUa -5' |
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12429 | 5' | -52 | NC_003324.1 | + | 36825 | 0.71 | 0.597666 |
Target: 5'- uCCACgGGCCUCGCAucGGAUGAUg--- -3' miRNA: 3'- -GGUG-CCGGAGUGUcuUCUGCUAgaua -5' |
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12429 | 5' | -52 | NC_003324.1 | + | 7133 | 0.79 | 0.222788 |
Target: 5'- gCGCGGCCUCGCGGAGGaaGCGAUa--- -3' miRNA: 3'- gGUGCCGGAGUGUCUUC--UGCUAgaua -5' |
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12429 | 5' | -52 | NC_003324.1 | + | 11690 | 1.09 | 0.002411 |
Target: 5'- gCCACGGCCUCACAGAAGACGAUCUAUa -3' miRNA: 3'- -GGUGCCGGAGUGUCUUCUGCUAGAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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