Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12430 | 3' | -57.5 | NC_003324.1 | + | 11941 | 0.66 | 0.604812 |
Target: 5'- gGGC--UGCUGuCAAUGCCccgguuGGGCGcGGCa -3' miRNA: 3'- gCCGaaACGAC-GUUGUGG------CCCGC-CUG- -5' |
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12430 | 3' | -57.5 | NC_003324.1 | + | 39799 | 0.66 | 0.593947 |
Target: 5'- uGGUg--GCUGCAGaACCGcaGGcCGGGCa -3' miRNA: 3'- gCCGaaaCGACGUUgUGGC--CC-GCCUG- -5' |
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12430 | 3' | -57.5 | NC_003324.1 | + | 36268 | 0.66 | 0.561574 |
Target: 5'- -uGCccUGCUGCuugacgaugauGGCAUCgGGGCGGGCg -3' miRNA: 3'- gcCGaaACGACG-----------UUGUGG-CCCGCCUG- -5' |
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12430 | 3' | -57.5 | NC_003324.1 | + | 56648 | 0.67 | 0.508823 |
Target: 5'- gCGGCguuauggGCgGCGGCAUgGGucGCGGGCa -3' miRNA: 3'- -GCCGaaa----CGaCGUUGUGgCC--CGCCUG- -5' |
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12430 | 3' | -57.5 | NC_003324.1 | + | 48723 | 0.67 | 0.507788 |
Target: 5'- uGGCUcaugccgaugaugUUGCgGCGGaGCCGGauGCGGGCg -3' miRNA: 3'- gCCGA-------------AACGaCGUUgUGGCC--CGCCUG- -5' |
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12430 | 3' | -57.5 | NC_003324.1 | + | 32801 | 0.68 | 0.478185 |
Target: 5'- uGGCUcuacgaGCUGUucGGCGCauGGCGGGCa -3' miRNA: 3'- gCCGAaa----CGACG--UUGUGgcCCGCCUG- -5' |
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12430 | 3' | -57.5 | NC_003324.1 | + | 53807 | 0.68 | 0.478185 |
Target: 5'- uGGUgggGCUGCAccaaggucuCGCCGGGCuGcGACa -3' miRNA: 3'- gCCGaaaCGACGUu--------GUGGCCCG-C-CUG- -5' |
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12430 | 3' | -57.5 | NC_003324.1 | + | 28688 | 0.68 | 0.458273 |
Target: 5'- gGGCcacGaggGCGACAgCGGGCGGAa -3' miRNA: 3'- gCCGaaaCga-CGUUGUgGCCCGCCUg -5' |
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12430 | 3' | -57.5 | NC_003324.1 | + | 29246 | 0.68 | 0.438812 |
Target: 5'- gCGGCUguucggGcCUGCAuCGCCGGGCucGCg -3' miRNA: 3'- -GCCGAaa----C-GACGUuGUGGCCCGccUG- -5' |
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12430 | 3' | -57.5 | NC_003324.1 | + | 29034 | 0.69 | 0.419832 |
Target: 5'- aCGaGCgcUUGCUcaaGCAGCGCCaGGCGGcACg -3' miRNA: 3'- -GC-CGa-AACGA---CGUUGUGGcCCGCC-UG- -5' |
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12430 | 3' | -57.5 | NC_003324.1 | + | 44632 | 0.69 | 0.401357 |
Target: 5'- uGGCUgacUGCccaUGCGAUGCCGGGUGucCa -3' miRNA: 3'- gCCGAa--ACG---ACGUUGUGGCCCGCcuG- -5' |
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12430 | 3' | -57.5 | NC_003324.1 | + | 50793 | 0.69 | 0.391419 |
Target: 5'- aCGGCaaUGCUGCcACACCGGaGCagugccaGGAg -3' miRNA: 3'- -GCCGaaACGACGuUGUGGCC-CG-------CCUg -5' |
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12430 | 3' | -57.5 | NC_003324.1 | + | 21093 | 0.7 | 0.374636 |
Target: 5'- gCGGCgcgUGCUGCGucggcuuccACGUCGGGCuGGCg -3' miRNA: 3'- -GCCGaa-ACGACGU---------UGUGGCCCGcCUG- -5' |
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12430 | 3' | -57.5 | NC_003324.1 | + | 40419 | 0.71 | 0.317122 |
Target: 5'- cCGGCaagacUGCUGCAGCGCCgaaGGGUgcaacuaagcaGGGCa -3' miRNA: 3'- -GCCGaa---ACGACGUUGUGG---CCCG-----------CCUG- -5' |
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12430 | 3' | -57.5 | NC_003324.1 | + | 54576 | 0.71 | 0.302703 |
Target: 5'- aGGCUUcgcggaUGCcccucacccauuccgGguGCAUCGGGCGGGCa -3' miRNA: 3'- gCCGAA------ACGa--------------CguUGUGGCCCGCCUG- -5' |
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12430 | 3' | -57.5 | NC_003324.1 | + | 16161 | 0.72 | 0.280272 |
Target: 5'- aGGCaucGCUG--GCACCGaGGCGGGCa -3' miRNA: 3'- gCCGaaaCGACguUGUGGC-CCGCCUG- -5' |
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12430 | 3' | -57.5 | NC_003324.1 | + | 39651 | 0.72 | 0.266514 |
Target: 5'- aGGCUggUGCUGacguGGCGCCaGGCgGGACg -3' miRNA: 3'- gCCGAa-ACGACg---UUGUGGcCCG-CCUG- -5' |
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12430 | 3' | -57.5 | NC_003324.1 | + | 9663 | 0.73 | 0.216901 |
Target: 5'- aCGcGCUggGCgGCGugGgGCCGGGCGGAUa -3' miRNA: 3'- -GC-CGAaaCGaCGU--UgUGGCCCGCCUG- -5' |
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12430 | 3' | -57.5 | NC_003324.1 | + | 12020 | 1.1 | 0.000469 |
Target: 5'- cCGGCUUUGCUGCAACACCGGGCGGACg -3' miRNA: 3'- -GCCGAAACGACGUUGUGGCCCGCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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