Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12432 | 5' | -54.2 | NC_003324.1 | + | 50434 | 0.66 | 0.74541 |
Target: 5'- ---cGCuGGGcgUGgUGCAGCUCgcagGGCu -3' miRNA: 3'- uagaCGuCCUuaACgACGUCGAG----CCG- -5' |
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12432 | 5' | -54.2 | NC_003324.1 | + | 44814 | 0.66 | 0.71273 |
Target: 5'- -gCUGCGGGGAgaagGggGCGGCgucaUUGGCg -3' miRNA: 3'- uaGACGUCCUUaa--CgaCGUCG----AGCCG- -5' |
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12432 | 5' | -54.2 | NC_003324.1 | + | 46484 | 0.66 | 0.690503 |
Target: 5'- -aCUGCAGGAcuaccgcaaGCgccucaaggGUAGCUUGGCu -3' miRNA: 3'- uaGACGUCCUuaa------CGa--------CGUCGAGCCG- -5' |
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12432 | 5' | -54.2 | NC_003324.1 | + | 26192 | 0.67 | 0.679293 |
Target: 5'- -gCUGCGGGGcugcGuCUGCGucguccccucGCUCGGCa -3' miRNA: 3'- uaGACGUCCUuaa-C-GACGU----------CGAGCCG- -5' |
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12432 | 5' | -54.2 | NC_003324.1 | + | 16261 | 0.67 | 0.644295 |
Target: 5'- uUCUgGCGGGA---GCUGCGGUgcccgccUCGGUg -3' miRNA: 3'- uAGA-CGUCCUuaaCGACGUCG-------AGCCG- -5' |
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12432 | 5' | -54.2 | NC_003324.1 | + | 29833 | 0.68 | 0.611444 |
Target: 5'- cAUCUGCAGGcGUggaaGCUGCuGUg-GGCu -3' miRNA: 3'- -UAGACGUCCuUAa---CGACGuCGagCCG- -5' |
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12432 | 5' | -54.2 | NC_003324.1 | + | 27592 | 0.68 | 0.60014 |
Target: 5'- aAUUUGCgAGGGuuuUUGCUGCGcGUcCGGCu -3' miRNA: 3'- -UAGACG-UCCUu--AACGACGU-CGaGCCG- -5' |
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12432 | 5' | -54.2 | NC_003324.1 | + | 34622 | 0.68 | 0.577629 |
Target: 5'- cUCUGCAGu--UUGC-GCAcCUCGGCg -3' miRNA: 3'- uAGACGUCcuuAACGaCGUcGAGCCG- -5' |
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12432 | 5' | -54.2 | NC_003324.1 | + | 35333 | 0.69 | 0.537635 |
Target: 5'- -gCUGCGGuugcuGUUGCUGCGGUugcuguugugccuggUCGGCc -3' miRNA: 3'- uaGACGUCcu---UAACGACGUCG---------------AGCCG- -5' |
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12432 | 5' | -54.2 | NC_003324.1 | + | 16515 | 0.69 | 0.533247 |
Target: 5'- gAUgUGCAGcGGAUcgGCcugUGCGuGCUCGGCg -3' miRNA: 3'- -UAgACGUC-CUUAa-CG---ACGU-CGAGCCG- -5' |
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12432 | 5' | -54.2 | NC_003324.1 | + | 31099 | 0.71 | 0.448728 |
Target: 5'- -cUUGCGGcAAUgcGCUGCuGCUCGGCu -3' miRNA: 3'- uaGACGUCcUUAa-CGACGuCGAGCCG- -5' |
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12432 | 5' | -54.2 | NC_003324.1 | + | 11664 | 0.72 | 0.390448 |
Target: 5'- aGUCUccgGCAGGAGccGCgggaGCAGCaUCGGCu -3' miRNA: 3'- -UAGA---CGUCCUUaaCGa---CGUCG-AGCCG- -5' |
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12432 | 5' | -54.2 | NC_003324.1 | + | 13388 | 1.09 | 0.000952 |
Target: 5'- gAUCUGCAGGAAUUGCUGCAGCUCGGCc -3' miRNA: 3'- -UAGACGUCCUUAACGACGUCGAGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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