Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12433 | 3' | -53.7 | NC_003324.1 | + | 14316 | 1.1 | 0.001233 |
Target: 5'- aCUACGGCAGUGAAGCACGUCAGAGCGa -3' miRNA: 3'- -GAUGCCGUCACUUCGUGCAGUCUCGC- -5' |
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12433 | 3' | -53.7 | NC_003324.1 | + | 45831 | 0.72 | 0.476023 |
Target: 5'- gCUGCGGCgagaacAGUGAGGUggaaACGgcgCGGGGCGc -3' miRNA: 3'- -GAUGCCG------UCACUUCG----UGCa--GUCUCGC- -5' |
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12433 | 3' | -53.7 | NC_003324.1 | + | 6908 | 0.69 | 0.649591 |
Target: 5'- --gUGGCAGcGAuagAGCACGUagucgaGGAGCGg -3' miRNA: 3'- gauGCCGUCaCU---UCGUGCAg-----UCUCGC- -5' |
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12433 | 3' | -53.7 | NC_003324.1 | + | 1403 | 0.69 | 0.605109 |
Target: 5'- ---aGGgAGUcGAGCACGUCGGAGgCGu -3' miRNA: 3'- gaugCCgUCAcUUCGUGCAGUCUC-GC- -5' |
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12433 | 3' | -53.7 | NC_003324.1 | + | 17692 | 0.68 | 0.704735 |
Target: 5'- -gGCGGCAucGUGcucaacGGCGCGcCGGGGCa -3' miRNA: 3'- gaUGCCGU--CACu-----UCGUGCaGUCUCGc -5' |
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12433 | 3' | -53.7 | NC_003324.1 | + | 8735 | 0.67 | 0.715592 |
Target: 5'- -gGCGGCGGUGucgGAGaa-GUCGGuGCGa -3' miRNA: 3'- gaUGCCGUCAC---UUCgugCAGUCuCGC- -5' |
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12433 | 3' | -53.7 | NC_003324.1 | + | 49869 | 0.67 | 0.712343 |
Target: 5'- gUACGGCaAGUGAgccGGCgggcaaagaucgcuGCGUCGGcGCGc -3' miRNA: 3'- gAUGCCG-UCACU---UCG--------------UGCAGUCuCGC- -5' |
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12433 | 3' | -53.7 | NC_003324.1 | + | 21270 | 0.67 | 0.715592 |
Target: 5'- ---aGGCGGUuGAGCACGaUCGcGAGCa -3' miRNA: 3'- gaugCCGUCAcUUCGUGC-AGU-CUCGc -5' |
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12433 | 3' | -53.7 | NC_003324.1 | + | 26920 | 0.67 | 0.737042 |
Target: 5'- -gGCGGCGaucgacGAGGCACucagCGGAGCGg -3' miRNA: 3'- gaUGCCGUca----CUUCGUGca--GUCUCGC- -5' |
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12433 | 3' | -53.7 | NC_003324.1 | + | 38071 | 0.66 | 0.808099 |
Target: 5'- -cGCGGUacugcgccuucuGGcGAAGCGCGUCGGcGCc -3' miRNA: 3'- gaUGCCG------------UCaCUUCGUGCAGUCuCGc -5' |
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12433 | 3' | -53.7 | NC_003324.1 | + | 1530 | 0.66 | 0.788571 |
Target: 5'- -gGCGGCAGUGguGCGCGgcgCuauGGCc -3' miRNA: 3'- gaUGCCGUCACuuCGUGCa--Guc-UCGc -5' |
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12433 | 3' | -53.7 | NC_003324.1 | + | 6131 | 0.66 | 0.813814 |
Target: 5'- -gAUGGCAG-GAugcuggacccuaagAGCAUGUCgucggcaGGAGCGg -3' miRNA: 3'- gaUGCCGUCaCU--------------UCGUGCAG-------UCUCGC- -5' |
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12433 | 3' | -53.7 | NC_003324.1 | + | 23484 | 0.66 | 0.817585 |
Target: 5'- -cGCGGCcaacgagaaAGccGAGCGCGUCAaAGCGg -3' miRNA: 3'- gaUGCCG---------UCacUUCGUGCAGUcUCGC- -5' |
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12433 | 3' | -53.7 | NC_003324.1 | + | 29713 | 0.66 | 0.808099 |
Target: 5'- -gGCGGCGGcguugcgccaGAAGCAgG-CAGAGCc -3' miRNA: 3'- gaUGCCGUCa---------CUUCGUgCaGUCUCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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