Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12433 | 5' | -60.6 | NC_003324.1 | + | 19465 | 0.71 | 0.232793 |
Target: 5'- cGGCGCGacgcgcGCCGGCGUcgAUCCAUcGCUGa -3' miRNA: 3'- -UCGCGCc-----CGGCUGCA--UAGGUGcCGAC- -5' |
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12433 | 5' | -60.6 | NC_003324.1 | + | 36525 | 0.69 | 0.312282 |
Target: 5'- cGcCGCGGGCgCGACGgcaugccCUACGGCa- -3' miRNA: 3'- uC-GCGCCCG-GCUGCaua----GGUGCCGac -5' |
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12433 | 5' | -60.6 | NC_003324.1 | + | 6217 | 0.68 | 0.327376 |
Target: 5'- cGGCGCGGGUcaguccgcuauCGGCGUAggCGCGGgUa -3' miRNA: 3'- -UCGCGCCCG-----------GCUGCAUagGUGCCgAc -5' |
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12433 | 5' | -60.6 | NC_003324.1 | + | 19609 | 0.68 | 0.335124 |
Target: 5'- uGCGCGugaagcGGCUGAUGgacgccuUCCGCGGCa- -3' miRNA: 3'- uCGCGC------CCGGCUGCau-----AGGUGCCGac -5' |
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12433 | 5' | -60.6 | NC_003324.1 | + | 54345 | 0.68 | 0.351018 |
Target: 5'- cGGCuCGGcGCUGAUGccgGUCCAUGGCa- -3' miRNA: 3'- -UCGcGCC-CGGCUGCa--UAGGUGCCGac -5' |
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12433 | 5' | -60.6 | NC_003324.1 | + | 53544 | 0.67 | 0.375846 |
Target: 5'- cGCGCGGguguacuuGCCGGCGUugaUACGGuCUGu -3' miRNA: 3'- uCGCGCC--------CGGCUGCAuagGUGCC-GAC- -5' |
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12433 | 5' | -60.6 | NC_003324.1 | + | 7394 | 0.67 | 0.401834 |
Target: 5'- cGCGCGGaaCGACGgucacgGUgUugGGCUGg -3' miRNA: 3'- uCGCGCCcgGCUGCa-----UAgGugCCGAC- -5' |
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12433 | 5' | -60.6 | NC_003324.1 | + | 40066 | 0.66 | 0.476391 |
Target: 5'- uGCGCGGGuCUGGCcggaaCGCGGCg- -3' miRNA: 3'- uCGCGCCC-GGCUGcauagGUGCCGac -5' |
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12433 | 5' | -60.6 | NC_003324.1 | + | 42910 | 0.66 | 0.457085 |
Target: 5'- cAGCGCGGGgCGACc-AUUgGCGGUc- -3' miRNA: 3'- -UCGCGCCCgGCUGcaUAGgUGCCGac -5' |
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12433 | 5' | -60.6 | NC_003324.1 | + | 23688 | 0.66 | 0.455177 |
Target: 5'- cGCGcCGGGCuucacauCGAUGUcgacugccucgauGUCCGcCGGCUGc -3' miRNA: 3'- uCGC-GCCCG-------GCUGCA-------------UAGGU-GCCGAC- -5' |
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12433 | 5' | -60.6 | NC_003324.1 | + | 44318 | 0.66 | 0.451375 |
Target: 5'- uGGCGC-GGCCGAUagccucugcagCCACGGCg- -3' miRNA: 3'- -UCGCGcCCGGCUGcaua-------GGUGCCGac -5' |
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12433 | 5' | -60.6 | NC_003324.1 | + | 1435 | 0.66 | 0.418873 |
Target: 5'- cAGCGCGacgcaaaggaaacGGCgCGACGUAUCCggcaGCGGa-- -3' miRNA: 3'- -UCGCGC-------------CCG-GCUGCAUAGG----UGCCgac -5' |
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12433 | 5' | -60.6 | NC_003324.1 | + | 2677 | 0.67 | 0.40985 |
Target: 5'- cAGCGCGguGGCCGcuucggcaagggcACGaUGUCgGCGGCg- -3' miRNA: 3'- -UCGCGC--CCGGC-------------UGC-AUAGgUGCCGac -5' |
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12433 | 5' | -60.6 | NC_003324.1 | + | 1509 | 0.67 | 0.401834 |
Target: 5'- cGGC-CGGGCCaGCGaggAUCCgGCGGCa- -3' miRNA: 3'- -UCGcGCCCGGcUGCa--UAGG-UGCCGac -5' |
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12433 | 5' | -60.6 | NC_003324.1 | + | 35478 | 0.68 | 0.343004 |
Target: 5'- gAGCGUGGGgaCGGCGaUcgCCGuacCGGCUGc -3' miRNA: 3'- -UCGCGCCCg-GCUGC-AuaGGU---GCCGAC- -5' |
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12433 | 5' | -60.6 | NC_003324.1 | + | 14544 | 0.69 | 0.304935 |
Target: 5'- uGGCGCucaaGGCCGACGUGgucaacaugggUCGCGGCc- -3' miRNA: 3'- -UCGCGc---CCGGCUGCAUa----------GGUGCCGac -5' |
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12433 | 5' | -60.6 | NC_003324.1 | + | 38824 | 0.71 | 0.210352 |
Target: 5'- cGUGCGGGUCcauGGCGUAUUCGuCGGCg- -3' miRNA: 3'- uCGCGCCCGG---CUGCAUAGGU-GCCGac -5' |
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12433 | 5' | -60.6 | NC_003324.1 | + | 14355 | 1.07 | 0.000484 |
Target: 5'- aAGCGCGGGCCGACGUAUCCACGGCUGc -3' miRNA: 3'- -UCGCGCCCGGCUGCAUAGGUGCCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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