Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12434 | 3' | -64.6 | NC_003324.1 | + | 53190 | 0.66 | 0.275889 |
Target: 5'- -uGUGGUgGGUGGUaGCuGGCGGccGGCu -3' miRNA: 3'- uuCGCCGgCCGCCG-CGuCCGCCa-UCG- -5' |
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12434 | 3' | -64.6 | NC_003324.1 | + | 9726 | 0.66 | 0.269309 |
Target: 5'- cAGCGcGuuGGCGuuGCcGGCGGUguuGGCc -3' miRNA: 3'- uUCGC-CggCCGCcgCGuCCGCCA---UCG- -5' |
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12434 | 3' | -64.6 | NC_003324.1 | + | 47707 | 0.66 | 0.262218 |
Target: 5'- uGGCGGcCCGGuCGccgucauGCGCGGcGUGGUaaAGCu -3' miRNA: 3'- uUCGCC-GGCC-GC-------CGCGUC-CGCCA--UCG- -5' |
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12434 | 3' | -64.6 | NC_003324.1 | + | 47987 | 0.67 | 0.24425 |
Target: 5'- cAGCGgauaaGCCGGCcaucGGCGCAGcaCGGUucGGCa -3' miRNA: 3'- uUCGC-----CGGCCG----CCGCGUCc-GCCA--UCG- -5' |
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12434 | 3' | -64.6 | NC_003324.1 | + | 12279 | 0.67 | 0.24425 |
Target: 5'- -cGCGagaccguCCGGCGGCucguuccaguccGCAGaCGGUAGCg -3' miRNA: 3'- uuCGCc------GGCCGCCG------------CGUCcGCCAUCG- -5' |
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12434 | 3' | -64.6 | NC_003324.1 | + | 10828 | 0.67 | 0.238295 |
Target: 5'- -uGauGUCGGUgaugacaacGGCGCuGGUGGUGGCu -3' miRNA: 3'- uuCgcCGGCCG---------CCGCGuCCGCCAUCG- -5' |
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12434 | 3' | -64.6 | NC_003324.1 | + | 28708 | 0.67 | 0.226751 |
Target: 5'- aAGGCGGCCcgcaacgauccgGGCcacgagGGCgacaGCGGGCGGaAGCc -3' miRNA: 3'- -UUCGCCGG------------CCG------CCG----CGUCCGCCaUCG- -5' |
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12434 | 3' | -64.6 | NC_003324.1 | + | 41539 | 0.67 | 0.21786 |
Target: 5'- -cGCGuGCCaaacgcuaacgacaGCGGCGCGGGCcuguuccucgcaGGUGGCc -3' miRNA: 3'- uuCGC-CGGc-------------CGCCGCGUCCG------------CCAUCG- -5' |
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12434 | 3' | -64.6 | NC_003324.1 | + | 48978 | 0.68 | 0.194936 |
Target: 5'- uGGCGGgUGGCGGaguccuggaUGCGGGCGaaGGCg -3' miRNA: 3'- uUCGCCgGCCGCC---------GCGUCCGCcaUCG- -5' |
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12434 | 3' | -64.6 | NC_003324.1 | + | 8412 | 0.68 | 0.188579 |
Target: 5'- -uGCGggucagggauuuccGCCGGCugcaGGUGCAGGCGGauuuGCg -3' miRNA: 3'- uuCGC--------------CGGCCG----CCGCGUCCGCCau--CG- -5' |
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12434 | 3' | -64.6 | NC_003324.1 | + | 25507 | 0.69 | 0.175956 |
Target: 5'- gGGGCGGCCuGCGcaaCGCcuucuacccgAGGaCGGUAGCg -3' miRNA: 3'- -UUCGCCGGcCGCc--GCG----------UCC-GCCAUCG- -5' |
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12434 | 3' | -64.6 | NC_003324.1 | + | 6221 | 0.69 | 0.158224 |
Target: 5'- cAGGCGGCgCGGgucaguccgcuauCGGCGUAGGCGcgGGUa -3' miRNA: 3'- -UUCGCCG-GCC-------------GCCGCGUCCGCcaUCG- -5' |
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12434 | 3' | -64.6 | NC_003324.1 | + | 3537 | 0.69 | 0.154151 |
Target: 5'- gGAGCGGaaCCGGCugcaaacGGCGCuu-CGGUGGCg -3' miRNA: 3'- -UUCGCC--GGCCG-------CCGCGuccGCCAUCG- -5' |
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12434 | 3' | -64.6 | NC_003324.1 | + | 14809 | 0.71 | 0.11557 |
Target: 5'- -cGCGGCCGGCaucGGCaGCuuccuGGGCGGcaucGGCg -3' miRNA: 3'- uuCGCCGGCCG---CCG-CG-----UCCGCCa---UCG- -5' |
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12434 | 3' | -64.6 | NC_003324.1 | + | 18990 | 0.71 | 0.11557 |
Target: 5'- cGAGCGGgaaauaCGGCGGCuGCAcGCGGcUGGCc -3' miRNA: 3'- -UUCGCCg-----GCCGCCG-CGUcCGCC-AUCG- -5' |
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12434 | 3' | -64.6 | NC_003324.1 | + | 13158 | 0.75 | 0.058559 |
Target: 5'- cGGCGGUCGGCGGC---GGCGGUaccGGCu -3' miRNA: 3'- uUCGCCGGCCGCCGcguCCGCCA---UCG- -5' |
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12434 | 3' | -64.6 | NC_003324.1 | + | 30146 | 0.79 | 0.028408 |
Target: 5'- cAGCGGCCggugucgcgaccGGCGGCGUgauGGGCGGcGGCa -3' miRNA: 3'- uUCGCCGG------------CCGCCGCG---UCCGCCaUCG- -5' |
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12434 | 3' | -64.6 | NC_003324.1 | + | 31317 | 0.89 | 0.004935 |
Target: 5'- -uGCGGCCGGCGGCGCuGGCGcUGGCg -3' miRNA: 3'- uuCGCCGGCCGCCGCGuCCGCcAUCG- -5' |
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12434 | 3' | -64.6 | NC_003324.1 | + | 14591 | 1.08 | 0.000126 |
Target: 5'- cAAGCGGCCGGCGGCGCAGGCGGUAGCg -3' miRNA: 3'- -UUCGCCGGCCGCCGCGUCCGCCAUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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