Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12434 | 5' | -54.6 | NC_003324.1 | + | 14571 | 0.66 | 0.754569 |
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Target: 5'- uGGGUcGCGGCCUg--CCGGCGcaagCGGc -3' miRNA: 3'- -CUCGaUGCCGGGaaaGGCCGUua--GUC- -5' |
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| 12434 | 5' | -54.6 | NC_003324.1 | + | 33828 | 0.66 | 0.73344 |
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Target: 5'- cGGCUugcACGGCCgccUCgCGGCAAUCGu -3' miRNA: 3'- cUCGA---UGCCGGgaaAG-GCCGUUAGUc -5' |
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| 12434 | 5' | -54.6 | NC_003324.1 | + | 16324 | 0.66 | 0.73344 |
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Target: 5'- -uGUUAgGGCCCUgcgCUGGCcgcGUCGGc -3' miRNA: 3'- cuCGAUgCCGGGAaa-GGCCGu--UAGUC- -5' |
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| 12434 | 5' | -54.6 | NC_003324.1 | + | 42916 | 0.66 | 0.711896 |
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Target: 5'- gGGGCgaccauugGCGGUCaCUgcgCCGGCGGUCu- -3' miRNA: 3'- -CUCGa-------UGCCGG-GAaa-GGCCGUUAGuc -5' |
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| 12434 | 5' | -54.6 | NC_003324.1 | + | 18103 | 0.68 | 0.61228 |
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Target: 5'- -cGC-AUGGCCCguggcgCCGGcCAAUCGGu -3' miRNA: 3'- cuCGaUGCCGGGaaa---GGCC-GUUAGUC- -5' |
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| 12434 | 5' | -54.6 | NC_003324.1 | + | 20788 | 0.71 | 0.422609 |
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Target: 5'- cAGCU-CGGCCCguggUCCGGCGcaaCGGa -3' miRNA: 3'- cUCGAuGCCGGGaa--AGGCCGUua-GUC- -5' |
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| 12434 | 5' | -54.6 | NC_003324.1 | + | 14627 | 1.09 | 0.001038 |
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Target: 5'- gGAGCUACGGCCCUUUCCGGCAAUCAGg -3' miRNA: 3'- -CUCGAUGCCGGGAAAGGCCGUUAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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