Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12435 | 5' | -57.5 | NC_003324.1 | + | 50959 | 0.66 | 0.63922 |
Target: 5'- -gGGGUGuCAGuca-UGGUCACCACCu -3' miRNA: 3'- agCCUACcGUCcuggACCAGUGGUGG- -5' |
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12435 | 5' | -57.5 | NC_003324.1 | + | 35035 | 0.66 | 0.628451 |
Target: 5'- gCGGcgGcaGCAGcGGCCUGaucgucGUCGCUGCCg -3' miRNA: 3'- aGCCuaC--CGUC-CUGGAC------CAGUGGUGG- -5' |
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12435 | 5' | -57.5 | NC_003324.1 | + | 52328 | 0.66 | 0.628451 |
Target: 5'- uUCGGc-GGCAGGACgaaCUGGcCGagugauagcCCGCCg -3' miRNA: 3'- -AGCCuaCCGUCCUG---GACCaGU---------GGUGG- -5' |
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12435 | 5' | -57.5 | NC_003324.1 | + | 8169 | 0.67 | 0.574822 |
Target: 5'- aCGGcgaccGUGGCA--GCCaUGGUCACCuCCu -3' miRNA: 3'- aGCC-----UACCGUccUGG-ACCAGUGGuGG- -5' |
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12435 | 5' | -57.5 | NC_003324.1 | + | 34906 | 0.67 | 0.55363 |
Target: 5'- gUCGGccGGCAGGAUCcacGUUGCCGgCg -3' miRNA: 3'- -AGCCuaCCGUCCUGGac-CAGUGGUgG- -5' |
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12435 | 5' | -57.5 | NC_003324.1 | + | 30110 | 0.67 | 0.55363 |
Target: 5'- gCGGcAUGGguGGugUUGGUgGCgCAgCa -3' miRNA: 3'- aGCC-UACCguCCugGACCAgUG-GUgG- -5' |
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12435 | 5' | -57.5 | NC_003324.1 | + | 2777 | 0.67 | 0.532676 |
Target: 5'- aUCGcccGAucUGGCAGGucuaccGCCUGGUCACaagGCUg -3' miRNA: 3'- -AGC---CU--ACCGUCC------UGGACCAGUGg--UGG- -5' |
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12435 | 5' | -57.5 | NC_003324.1 | + | 9397 | 0.68 | 0.501806 |
Target: 5'- uUCGGGUGGCAugcuugcuCUUGG-CACCACg -3' miRNA: 3'- -AGCCUACCGUccu-----GGACCaGUGGUGg -5' |
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12435 | 5' | -57.5 | NC_003324.1 | + | 32816 | 0.68 | 0.501806 |
Target: 5'- uUCGGcgcAUGGCGGGcagccGCCUcGaUCAUCGCCa -3' miRNA: 3'- -AGCC---UACCGUCC-----UGGAcC-AGUGGUGG- -5' |
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12435 | 5' | -57.5 | NC_003324.1 | + | 56644 | 0.69 | 0.433145 |
Target: 5'- uUCGGGUGGCGGccagaGACCUcaccucacGGUCACguUCc -3' miRNA: 3'- -AGCCUACCGUC-----CUGGA--------CCAGUGguGG- -5' |
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12435 | 5' | -57.5 | NC_003324.1 | + | 39324 | 0.69 | 0.414546 |
Target: 5'- aUCGc---GCAGGACCgGGUUACCGCg -3' miRNA: 3'- -AGCcuacCGUCCUGGaCCAGUGGUGg -5' |
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12435 | 5' | -57.5 | NC_003324.1 | + | 17548 | 0.7 | 0.405432 |
Target: 5'- cUCGGcgcgacGGCAGGACUUGGUgAgcagugguUCACCg -3' miRNA: 3'- -AGCCua----CCGUCCUGGACCAgU--------GGUGG- -5' |
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12435 | 5' | -57.5 | NC_003324.1 | + | 56307 | 0.71 | 0.32156 |
Target: 5'- aCGGAU-GCAGGAU---GUCGCCGCCg -3' miRNA: 3'- aGCCUAcCGUCCUGgacCAGUGGUGG- -5' |
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12435 | 5' | -57.5 | NC_003324.1 | + | 17307 | 1.13 | 0.000365 |
Target: 5'- aUCGGAUGGCAGGACCUGGUCACCACCg -3' miRNA: 3'- -AGCCUACCGUCCUGGACCAGUGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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