Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12438 | 3' | -55.1 | NC_003324.1 | + | 20005 | 0.69 | 0.551892 |
Target: 5'- aCCAUCAGCcaggUGUUGCUguggagcuccGugGCCuGAGc -3' miRNA: 3'- -GGUAGUCGa---GCAACGA----------CugCGGcCUC- -5' |
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12438 | 3' | -55.1 | NC_003324.1 | + | 26868 | 0.66 | 0.76672 |
Target: 5'- cCCuuuUCAgGCUCaagGgUGACGCCGGGc -3' miRNA: 3'- -GGu--AGU-CGAGcaaCgACUGCGGCCUc -5' |
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12438 | 3' | -55.1 | NC_003324.1 | + | 50447 | 0.66 | 0.75655 |
Target: 5'- ---gCAGCUCGcaggGCUGcGCGauCCGGAGg -3' miRNA: 3'- gguaGUCGAGCaa--CGAC-UGC--GGCCUC- -5' |
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12438 | 3' | -55.1 | NC_003324.1 | + | 54272 | 0.66 | 0.753474 |
Target: 5'- gCAUCAGCgccgagcCGUUGCUGgaaggccugcgucuGCGCCGu-- -3' miRNA: 3'- gGUAGUCGa------GCAACGAC--------------UGCGGCcuc -5' |
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12438 | 3' | -55.1 | NC_003324.1 | + | 25075 | 0.66 | 0.745219 |
Target: 5'- -gGUCAGCUucucaaacccgacUGgcgucuggaUGUUGGCGCCGGGGu -3' miRNA: 3'- ggUAGUCGA-------------GCa--------ACGACUGCGGCCUC- -5' |
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12438 | 3' | -55.1 | NC_003324.1 | + | 49834 | 0.66 | 0.725332 |
Target: 5'- gUCA-CAGCUCGUgccgcgGCUGGCaucaaGgCGGAGu -3' miRNA: 3'- -GGUaGUCGAGCAa-----CGACUG-----CgGCCUC- -5' |
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12438 | 3' | -55.1 | NC_003324.1 | + | 10183 | 0.66 | 0.714727 |
Target: 5'- cCCGUCAGCuUCGgcuagUGCUcaucGGCGCCa--- -3' miRNA: 3'- -GGUAGUCG-AGCa----ACGA----CUGCGGccuc -5' |
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12438 | 3' | -55.1 | NC_003324.1 | + | 57129 | 0.66 | 0.714727 |
Target: 5'- gCCAUgaAGCUCGaaacccUGCUGACGCCc--- -3' miRNA: 3'- -GGUAg-UCGAGCa-----ACGACUGCGGccuc -5' |
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12438 | 3' | -55.1 | NC_003324.1 | + | 56061 | 0.67 | 0.693286 |
Target: 5'- gCCGUCGGCUUccagGCUGGCGaacccgcgaUGGAGc -3' miRNA: 3'- -GGUAGUCGAGcaa-CGACUGCg--------GCCUC- -5' |
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12438 | 3' | -55.1 | NC_003324.1 | + | 21471 | 0.68 | 0.649791 |
Target: 5'- gCAUagAGCUCGUUGCcc-UGCCGGAu -3' miRNA: 3'- gGUAg-UCGAGCAACGacuGCGGCCUc -5' |
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12438 | 3' | -55.1 | NC_003324.1 | + | 38802 | 0.68 | 0.638851 |
Target: 5'- uCCuUCGGCUCGaUGaUGACGCCGugcGGGu -3' miRNA: 3'- -GGuAGUCGAGCaACgACUGCGGC---CUC- -5' |
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12438 | 3' | -55.1 | NC_003324.1 | + | 25607 | 0.68 | 0.595127 |
Target: 5'- uCUGUCGGC-CGcUGCUG-CGCCGGc- -3' miRNA: 3'- -GGUAGUCGaGCaACGACuGCGGCCuc -5' |
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12438 | 3' | -55.1 | NC_003324.1 | + | 18744 | 1.09 | 0.001085 |
Target: 5'- uCCAUCAGCUCGUUGCUGACGCCGGAGc -3' miRNA: 3'- -GGUAGUCGAGCAACGACUGCGGCCUC- -5' |
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12438 | 3' | -55.1 | NC_003324.1 | + | 47973 | 0.66 | 0.725332 |
Target: 5'- gCCAUCGGCgcagcacgguUCGgcaaaggucCUGACGCCGGu- -3' miRNA: 3'- -GGUAGUCG----------AGCaac------GACUGCGGCCuc -5' |
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12438 | 3' | -55.1 | NC_003324.1 | + | 31321 | 0.67 | 0.704041 |
Target: 5'- gCAgugCGGCcggCGgcGCUGGCGCUGGcGGg -3' miRNA: 3'- gGUa--GUCGa--GCaaCGACUGCGGCC-UC- -5' |
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12438 | 3' | -55.1 | NC_003324.1 | + | 43646 | 0.69 | 0.541222 |
Target: 5'- -aAUCGGCUgacguugcgcggCGUgugGCUGAUGCUGGAa -3' miRNA: 3'- ggUAGUCGA------------GCAa--CGACUGCGGCCUc -5' |
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12438 | 3' | -55.1 | NC_003324.1 | + | 54353 | 0.79 | 0.152811 |
Target: 5'- uCCAgcaaCGGCUCGgcGCUGAUGCCGGu- -3' miRNA: 3'- -GGUa---GUCGAGCaaCGACUGCGGCCuc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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