Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12439 | 5' | -57.8 | NC_003324.1 | + | 26102 | 0.66 | 0.52739 |
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Target: 5'- gGUGaCGCcauuGGCGCGcUCAgucaccccgaagUCGGGCCUa -3' miRNA: 3'- -UAC-GCGu---CCGCGC-AGUa-----------AGCUCGGAa -5' |
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| 12439 | 5' | -57.8 | NC_003324.1 | + | 25886 | 0.66 | 0.507273 |
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Target: 5'- -cGCGCAGGCGUG-CGUcUCGAcuaugacgguaccGCCa- -3' miRNA: 3'- uaCGCGUCCGCGCaGUA-AGCU-------------CGGaa -5' |
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| 12439 | 5' | -57.8 | NC_003324.1 | + | 5484 | 0.66 | 0.497857 |
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Target: 5'- -gGUGCGGGCGCcaUCuucCGAGCCg- -3' miRNA: 3'- uaCGCGUCCGCGc-AGuaaGCUCGGaa -5' |
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| 12439 | 5' | -57.8 | NC_003324.1 | + | 17180 | 0.66 | 0.487489 |
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Target: 5'- -gGCGCuGGCGCGUCugUCGgacgcGGCUUUc -3' miRNA: 3'- uaCGCGuCCGCGCAGuaAGC-----UCGGAA- -5' |
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| 12439 | 5' | -57.8 | NC_003324.1 | + | 54205 | 0.67 | 0.447093 |
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Target: 5'- aAUGCGCAGGCGCcaacuuUUCGAGgUUg -3' miRNA: 3'- -UACGCGUCCGCGcagu--AAGCUCgGAa -5' |
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| 12439 | 5' | -57.8 | NC_003324.1 | + | 18127 | 0.67 | 0.427604 |
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Target: 5'- -cGCGCAgcGGCGUG-UAUUCGAcGCCg- -3' miRNA: 3'- uaCGCGU--CCGCGCaGUAAGCU-CGGaa -5' |
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| 12439 | 5' | -57.8 | NC_003324.1 | + | 16314 | 0.68 | 0.41805 |
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Target: 5'- cUGCGCuGGcCGCGUCGgcaCG-GCCUg -3' miRNA: 3'- uACGCGuCC-GCGCAGUaa-GCuCGGAa -5' |
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| 12439 | 5' | -57.8 | NC_003324.1 | + | 8457 | 0.7 | 0.2842 |
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Target: 5'- -gGCGCAuGCGCGUCAaUCGAGaCUa -3' miRNA: 3'- uaCGCGUcCGCGCAGUaAGCUCgGAa -5' |
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| 12439 | 5' | -57.8 | NC_003324.1 | + | 23366 | 0.71 | 0.277064 |
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Target: 5'- cUGCGCAgcuaugacGGCGCG-CAUcUGAGCCUUc -3' miRNA: 3'- uACGCGU--------CCGCGCaGUAaGCUCGGAA- -5' |
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| 12439 | 5' | -57.8 | NC_003324.1 | + | 19033 | 1.03 | 0.001196 |
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Target: 5'- gAUGCGCAGGCGCGUCAUUCGAGCCUUu -3' miRNA: 3'- -UACGCGUCCGCGCAGUAAGCUCGGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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