Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12440 | 3' | -56.6 | NC_003324.1 | + | 19418 | 1.08 | 0.000786 |
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Target: 5'- uCGGCUAGAUGCUGCGCCUUGGCCUUGu -3' miRNA: 3'- -GCCGAUCUACGACGCGGAACCGGAAC- -5' |
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| 12440 | 3' | -56.6 | NC_003324.1 | + | 6304 | 0.71 | 0.318865 |
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Target: 5'- uGcGCUGc-UGCUGCGCCUcGGCCUUc -3' miRNA: 3'- gC-CGAUcuACGACGCGGAaCCGGAAc -5' |
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| 12440 | 3' | -56.6 | NC_003324.1 | + | 27702 | 0.69 | 0.44282 |
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Target: 5'- uGGC--GAUG-UGCGCCUcGGCCUUa -3' miRNA: 3'- gCCGauCUACgACGCGGAaCCGGAAc -5' |
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| 12440 | 3' | -56.6 | NC_003324.1 | + | 21207 | 0.69 | 0.452675 |
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Target: 5'- aGGCcgAGAUGCUGCGCaagcGCCa-- -3' miRNA: 3'- gCCGa-UCUACGACGCGgaacCGGaac -5' |
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| 12440 | 3' | -56.6 | NC_003324.1 | + | 5883 | 0.68 | 0.462647 |
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Target: 5'- cCGGCcuuggGGAUGacggcCUGCGCCUugaUGGCCc-- -3' miRNA: 3'- -GCCGa----UCUAC-----GACGCGGA---ACCGGaac -5' |
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| 12440 | 3' | -56.6 | NC_003324.1 | + | 1531 | 0.68 | 0.472734 |
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Target: 5'- gCGGCaguGGUGCgcgGCGCUaUGGCCc-- -3' miRNA: 3'- -GCCGau-CUACGa--CGCGGaACCGGaac -5' |
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| 12440 | 3' | -56.6 | NC_003324.1 | + | 30359 | 0.68 | 0.503633 |
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Target: 5'- gCGGCUuGG-GCgaccGUGCCUUGGCCc-- -3' miRNA: 3'- -GCCGAuCUaCGa---CGCGGAACCGGaac -5' |
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| 12440 | 3' | -56.6 | NC_003324.1 | + | 54321 | 0.68 | 0.514128 |
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Target: 5'- uGGCaaccGGUGCcGCGCCUUcGCUUUGa -3' miRNA: 3'- gCCGau--CUACGaCGCGGAAcCGGAAC- -5' |
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| 12440 | 3' | -56.6 | NC_003324.1 | + | 43994 | 0.66 | 0.633813 |
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Target: 5'- gGGCUcGGUGCcgGCGCCaaaGGCUUc- -3' miRNA: 3'- gCCGAuCUACGa-CGCGGaa-CCGGAac -5' |
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| 12440 | 3' | -56.6 | NC_003324.1 | + | 57327 | 0.66 | 0.633813 |
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Target: 5'- aGGCUGGccGCuUGCGacCCUUGGCg--- -3' miRNA: 3'- gCCGAUCuaCG-ACGC--GGAACCGgaac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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