Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12440 | 5' | -48.9 | NC_003324.1 | + | 21085 | 0.66 | 0.97305 |
Target: 5'- cGGCc--GCCGcGGCGCGUGcUGcgUCGg -3' miRNA: 3'- -CCGuuuCGGCuCUGCGCAC-AUaaAGU- -5' |
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12440 | 5' | -48.9 | NC_003324.1 | + | 17259 | 0.66 | 0.969886 |
Target: 5'- aGGCGugAAGCCGAG-CGCcUGUGc---- -3' miRNA: 3'- -CCGU--UUCGGCUCuGCGcACAUaaagu -5' |
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12440 | 5' | -48.9 | NC_003324.1 | + | 12087 | 0.66 | 0.96278 |
Target: 5'- aGCAAAGCCGgAGA-GCGUGaccgUCu -3' miRNA: 3'- cCGUUUCGGC-UCUgCGCACauaaAGu -5' |
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12440 | 5' | -48.9 | NC_003324.1 | + | 44077 | 0.67 | 0.950059 |
Target: 5'- cGGCGGcAGCCGAGGaggaaugaGCGaUGaAUUUCGc -3' miRNA: 3'- -CCGUU-UCGGCUCUg-------CGC-ACaUAAAGU- -5' |
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12440 | 5' | -48.9 | NC_003324.1 | + | 27685 | 0.68 | 0.934727 |
Target: 5'- cGGUAAAGCCG-GACGCGc-------- -3' miRNA: 3'- -CCGUUUCGGCuCUGCGCacauaaagu -5' |
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12440 | 5' | -48.9 | NC_003324.1 | + | 48702 | 0.68 | 0.910097 |
Target: 5'- cGGCGGAGCCG-GAUGCGg-------- -3' miRNA: 3'- -CCGUUUCGGCuCUGCGCacauaaagu -5' |
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12440 | 5' | -48.9 | NC_003324.1 | + | 29182 | 0.69 | 0.888509 |
Target: 5'- gGGCGAGGUCguaGAGAUGCGcGUGcUUCu -3' miRNA: 3'- -CCGUUUCGG---CUCUGCGCaCAUaAAGu -5' |
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12440 | 5' | -48.9 | NC_003324.1 | + | 32171 | 0.7 | 0.855786 |
Target: 5'- cGGCGacGAGCCGAGGCGCa--------- -3' miRNA: 3'- -CCGU--UUCGGCUCUGCGcacauaaagu -5' |
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12440 | 5' | -48.9 | NC_003324.1 | + | 12415 | 0.7 | 0.850514 |
Target: 5'- cGGCAaugccgacaaugucgGAGCCGAcguuGCGCGaGUAUUUCu -3' miRNA: 3'- -CCGU---------------UUCGGCUc---UGCGCaCAUAAAGu -5' |
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12440 | 5' | -48.9 | NC_003324.1 | + | 34150 | 0.71 | 0.809252 |
Target: 5'- gGGCGGAGCCGcuggcauuGGGCGcCGUGgGUaUCAu -3' miRNA: 3'- -CCGUUUCGGC--------UCUGC-GCACaUAaAGU- -5' |
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12440 | 5' | -48.9 | NC_003324.1 | + | 19452 | 1.13 | 0.002763 |
Target: 5'- cGGCAAAGCCGAGACGCGUGUAUUUCAa -3' miRNA: 3'- -CCGUUUCGGCUCUGCGCACAUAAAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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