Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12442 | 3' | -55.5 | NC_003324.1 | + | 18689 | 0.66 | 0.775007 |
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Target: 5'- -gCGcUUGCG-UGCggaAAGGUCGGGCa -3' miRNA: 3'- ggGC-AGCGCaACGag-UUCCAGCCUGc -5' |
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| 12442 | 3' | -55.5 | NC_003324.1 | + | 7850 | 0.66 | 0.774024 |
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Target: 5'- gCCCGUUGCGaucGCgccucccugCGAGGcgugcucUCGGACa -3' miRNA: 3'- -GGGCAGCGCaa-CGa--------GUUCC-------AGCCUGc -5' |
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| 12442 | 3' | -55.5 | NC_003324.1 | + | 10065 | 0.66 | 0.754077 |
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Target: 5'- aCCCGUgGCGUUGCUgGcuuGGUCcaugcuuGGcucGCGg -3' miRNA: 3'- -GGGCAgCGCAACGAgUu--CCAG-------CC---UGC- -5' |
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| 12442 | 3' | -55.5 | NC_003324.1 | + | 19396 | 0.66 | 0.734685 |
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Target: 5'- gCgCGUCGCGccgUGCaUGAGGUCGG-Cu -3' miRNA: 3'- -GgGCAGCGCa--ACGaGUUCCAGCCuGc -5' |
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| 12442 | 3' | -55.5 | NC_003324.1 | + | 36695 | 0.67 | 0.70337 |
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Target: 5'- aCCCGcuaUCGCGcggGCaCAAGGUCGcGAuCGa -3' miRNA: 3'- -GGGC---AGCGCaa-CGaGUUCCAGC-CU-GC- -5' |
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| 12442 | 3' | -55.5 | NC_003324.1 | + | 55025 | 0.68 | 0.649984 |
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Target: 5'- -gUGUCGCGccggGCaggCAAGG-CGGACGg -3' miRNA: 3'- ggGCAGCGCaa--CGa--GUUCCaGCCUGC- -5' |
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| 12442 | 3' | -55.5 | NC_003324.1 | + | 21918 | 0.68 | 0.649984 |
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Target: 5'- gCCCGgcugCGCGaacugaUGCagAAGG-CGGACGa -3' miRNA: 3'- -GGGCa---GCGCa-----ACGagUUCCaGCCUGC- -5' |
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| 12442 | 3' | -55.5 | NC_003324.1 | + | 53184 | 0.7 | 0.501806 |
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Target: 5'- aCCGUCugGCcgUGCUCAAGGagCGGACc -3' miRNA: 3'- gGGCAG--CGcaACGAGUUCCa-GCCUGc -5' |
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| 12442 | 3' | -55.5 | NC_003324.1 | + | 21502 | 1.11 | 0.000879 |
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Target: 5'- gCCCGUCGCGUUGCUCAAGGUCGGACGg -3' miRNA: 3'- -GGGCAGCGCAACGAGUUCCAGCCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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