Results 1 - 20 of 37 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12443 | 5' | -57.2 | NC_003324.1 | + | 1523 | 0.72 | 0.281694 |
Target: 5'- aGGAuCCGGCggcaGUGGUgcgcggCGCUAUGGCCCc -3' miRNA: 3'- aCCUcGGCCG----UAUCA------GCGGUAUCGGG- -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 3377 | 0.68 | 0.470035 |
Target: 5'- cGGcAGCCuGGUGUAGUC-CCAUuuCCCg -3' miRNA: 3'- aCC-UCGG-CCGUAUCAGcGGUAucGGG- -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 4845 | 0.68 | 0.480059 |
Target: 5'- aUGGcGCCGGCAacaacGUUgcgGCCGgucgAGCCCu -3' miRNA: 3'- -ACCuCGGCCGUau---CAG---CGGUa---UCGGG- -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 4978 | 0.66 | 0.59588 |
Target: 5'- cGGAGCCGaaaGCGauaaCGCCAgccugacGGCCCa -3' miRNA: 3'- aCCUCGGC---CGUaucaGCGGUa------UCGGG- -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 5545 | 0.67 | 0.552814 |
Target: 5'- gUGGGGCgGGCGacGcCGCCu--GCCUa -3' miRNA: 3'- -ACCUCGgCCGUauCaGCGGuauCGGG- -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 5889 | 0.66 | 0.628523 |
Target: 5'- uUGGGGaugaCGGCcug--CGCCuugAUGGCCCg -3' miRNA: 3'- -ACCUCg---GCCGuaucaGCGG---UAUCGGG- -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 11088 | 0.66 | 0.59588 |
Target: 5'- cGGuGCCaacuugaacGGCAacaccuacaaUGGccugCGCUAUGGCCCg -3' miRNA: 3'- aCCuCGG---------CCGU----------AUCa---GCGGUAUCGGG- -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 14472 | 0.67 | 0.552814 |
Target: 5'- cGGAccuuGCUGGCAccGUCGCgGaAGCCg -3' miRNA: 3'- aCCU----CGGCCGUauCAGCGgUaUCGGg -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 14520 | 0.72 | 0.267889 |
Target: 5'- uUGGAcGCCGGCAUGGgC-CCAgAGCCg -3' miRNA: 3'- -ACCU-CGGCCGUAUCaGcGGUaUCGGg -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 16061 | 0.68 | 0.470035 |
Target: 5'- aUGGcaucGCCGcGCAUcuuaucGUCGCCAacGCCCa -3' miRNA: 3'- -ACCu---CGGC-CGUAu-----CAGCGGUauCGGG- -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 16134 | 0.71 | 0.326476 |
Target: 5'- cGcGGCCGGCA-AGUCGUCGaacuUGGCCUc -3' miRNA: 3'- aCcUCGGCCGUaUCAGCGGU----AUCGGG- -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 16557 | 0.68 | 0.484097 |
Target: 5'- aGGGGCUGGCugcucaucgucUCGCCGUAGUUg -3' miRNA: 3'- aCCUCGGCCGuauc-------AGCGGUAUCGGg -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 18900 | 0.68 | 0.467049 |
Target: 5'- aUGGAGaCUGGCGccgaccuucgccgCGCCAUGGaCCCg -3' miRNA: 3'- -ACCUC-GGCCGUauca---------GCGGUAUC-GGG- -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 20408 | 0.67 | 0.5678 |
Target: 5'- cGGAGCCGuCAUcGUCGaucgcaagcagguacUCAUGGCCg -3' miRNA: 3'- aCCUCGGCcGUAuCAGC---------------GGUAUCGGg -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 20589 | 0.66 | 0.617626 |
Target: 5'- gGGAuCCGGUgcuGUGG-CGCCGUcGCCa -3' miRNA: 3'- aCCUcGGCCG---UAUCaGCGGUAuCGGg -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 21980 | 1.12 | 0.000363 |
Target: 5'- uUGGAGCCGGCAUAGUCGCCAUAGCCCg -3' miRNA: 3'- -ACCUCGGCCGUAUCAGCGGUAUCGGG- -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 24405 | 0.67 | 0.562436 |
Target: 5'- aGGAccgcguuGCCGGCAUGGUgcgcguccgcgaCGCCuucGCCa -3' miRNA: 3'- aCCU-------CGGCCGUAUCA------------GCGGuauCGGg -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 27228 | 0.67 | 0.563507 |
Target: 5'- aGGaAGUgGGCGgccaacGUCGCCAacgGGCUCa -3' miRNA: 3'- aCC-UCGgCCGUau----CAGCGGUa--UCGGG- -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 27632 | 0.68 | 0.470035 |
Target: 5'- cGuGAGCCGGCA-----GCCG-AGCCCg -3' miRNA: 3'- aC-CUCGGCCGUaucagCGGUaUCGGG- -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 28992 | 0.66 | 0.617626 |
Target: 5'- aGGAGCCGGaaucGGUCGCagcgaaggaCGaGGCCg -3' miRNA: 3'- aCCUCGGCCgua-UCAGCG---------GUaUCGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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