Results 21 - 37 of 37 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12443 | 5' | -57.2 | NC_003324.1 | + | 47198 | 0.68 | 0.470035 |
Target: 5'- aGGGGgUGGUGcAGUCGCCAgacuuGCCg -3' miRNA: 3'- aCCUCgGCCGUaUCAGCGGUau---CGGg -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 16061 | 0.68 | 0.470035 |
Target: 5'- aUGGcaucGCCGcGCAUcuuaucGUCGCCAacGCCCa -3' miRNA: 3'- -ACCu---CGGC-CGUAu-----CAGCGGUauCGGG- -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 27632 | 0.68 | 0.470035 |
Target: 5'- cGuGAGCCGGCA-----GCCG-AGCCCg -3' miRNA: 3'- aC-CUCGGCCGUaucagCGGUaUCGGG- -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 3377 | 0.68 | 0.470035 |
Target: 5'- cGGcAGCCuGGUGUAGUC-CCAUuuCCCg -3' miRNA: 3'- aCC-UCGG-CCGUAUCAGcGGUAucGGG- -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 18900 | 0.68 | 0.467049 |
Target: 5'- aUGGAGaCUGGCGccgaccuucgccgCGCCAUGGaCCCg -3' miRNA: 3'- -ACCUC-GGCCGUauca---------GCGGUAUC-GGG- -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 54264 | 0.69 | 0.421615 |
Target: 5'- aUGGA-CCGGCAUcAG-CGCCG-AGCCg -3' miRNA: 3'- -ACCUcGGCCGUA-UCaGCGGUaUCGGg -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 34147 | 0.7 | 0.391353 |
Target: 5'- cGGAGCCgcuGGCAuUGGgCGCCGUGGguaucauucuacacCCCa -3' miRNA: 3'- aCCUCGG---CCGU-AUCaGCGGUAUC--------------GGG- -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 31165 | 0.7 | 0.385118 |
Target: 5'- cGGAGCugccCGGCcug--CGCCAUcaGGCCCg -3' miRNA: 3'- aCCUCG----GCCGuaucaGCGGUA--UCGGG- -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 38356 | 0.7 | 0.376325 |
Target: 5'- cGGGG-CGGCGcUGGUCGCCGUcGUgCg -3' miRNA: 3'- aCCUCgGCCGU-AUCAGCGGUAuCGgG- -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 43245 | 0.7 | 0.350772 |
Target: 5'- gGGGGCCGccuGCAgGGUCGCC-UGGCa- -3' miRNA: 3'- aCCUCGGC---CGUaUCAGCGGuAUCGgg -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 30272 | 0.71 | 0.334434 |
Target: 5'- aUGcGGGCCaaGGCAcGGUCGCCcaAGCCg -3' miRNA: 3'- -AC-CUCGG--CCGUaUCAGCGGuaUCGGg -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 16134 | 0.71 | 0.326476 |
Target: 5'- cGcGGCCGGCA-AGUCGUCGaacuUGGCCUc -3' miRNA: 3'- aCcUCGGCCGUaUCAGCGGU----AUCGGG- -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 54825 | 0.72 | 0.30345 |
Target: 5'- gGuGAGaCCGGCAgggAGaUUGCCGcAGCCCu -3' miRNA: 3'- aC-CUC-GGCCGUa--UC-AGCGGUaUCGGG- -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 53702 | 0.72 | 0.281694 |
Target: 5'- uUGGAGCCGGCGacguucGUCGCgc-AGCUCg -3' miRNA: 3'- -ACCUCGGCCGUau----CAGCGguaUCGGG- -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 1523 | 0.72 | 0.281694 |
Target: 5'- aGGAuCCGGCggcaGUGGUgcgcggCGCUAUGGCCCc -3' miRNA: 3'- aCCUcGGCCG----UAUCA------GCGGUAUCGGG- -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 14520 | 0.72 | 0.267889 |
Target: 5'- uUGGAcGCCGGCAUGGgC-CCAgAGCCg -3' miRNA: 3'- -ACCU-CGGCCGUAUCaGcGGUaUCGGg -5' |
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12443 | 5' | -57.2 | NC_003324.1 | + | 21980 | 1.12 | 0.000363 |
Target: 5'- uUGGAGCCGGCAUAGUCGCCAUAGCCCg -3' miRNA: 3'- -ACCUCGGCCGUAUCAGCGGUAUCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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