Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12444 | 5' | -60.5 | NC_003324.1 | + | 38096 | 0.66 | 0.455294 |
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Target: 5'- cGcGUCGGCGccGUGCuGGUgACCGUUCUu -3' miRNA: 3'- aC-CAGCCGCu-CGCG-CCA-UGGCGAGAc -5' |
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| 12444 | 5' | -60.5 | NC_003324.1 | + | 24779 | 0.66 | 0.44299 |
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Target: 5'- aGGUCGGCGAGUGgaaGGgcaagGauggcaaggcugucCCGCUCa- -3' miRNA: 3'- aCCAGCCGCUCGCg--CCa----U--------------GGCGAGac -5' |
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| 12444 | 5' | -60.5 | NC_003324.1 | + | 47699 | 0.67 | 0.408138 |
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Target: 5'- cGGUCGccgucauGCGcGGCGUGGUAaaGCUCg- -3' miRNA: 3'- aCCAGC-------CGC-UCGCGCCAUggCGAGac -5' |
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| 12444 | 5' | -60.5 | NC_003324.1 | + | 54002 | 0.67 | 0.391367 |
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Target: 5'- aGG-CGGCGaAGCGCacuUGCCGCgCUGa -3' miRNA: 3'- aCCaGCCGC-UCGCGcc-AUGGCGaGAC- -5' |
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| 12444 | 5' | -60.5 | NC_003324.1 | + | 1073 | 0.67 | 0.374206 |
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Target: 5'- -aGUCcuGGCG-GCuGCGGUACCGCUUc- -3' miRNA: 3'- acCAG--CCGCuCG-CGCCAUGGCGAGac -5' |
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| 12444 | 5' | -60.5 | NC_003324.1 | + | 20590 | 0.68 | 0.349437 |
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Target: 5'- cGGUCGGCGGGCaCGcccUCGCUCa- -3' miRNA: 3'- aCCAGCCGCUCGcGCcauGGCGAGac -5' |
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| 12444 | 5' | -60.5 | NC_003324.1 | + | 35471 | 0.7 | 0.27551 |
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Target: 5'- -uGUCGGCGAGCGUGGggACgGCg--- -3' miRNA: 3'- acCAGCCGCUCGCGCCa-UGgCGagac -5' |
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| 12444 | 5' | -60.5 | NC_003324.1 | + | 48059 | 0.7 | 0.268848 |
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Target: 5'- gGGUCaGUcAGCGCgucGGUGCCGgUCUGg -3' miRNA: 3'- aCCAGcCGcUCGCG---CCAUGGCgAGAC- -5' |
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| 12444 | 5' | -60.5 | NC_003324.1 | + | 13155 | 0.78 | 0.077649 |
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Target: 5'- cGGUCGGCG-GCgGCGGUACCGg-CUGc -3' miRNA: 3'- aCCAGCCGCuCG-CGCCAUGGCgaGAC- -5' |
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| 12444 | 5' | -60.5 | NC_003324.1 | + | 23181 | 1.07 | 0.000465 |
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Target: 5'- aUGGUCGGCGAGCGCGGUACCGCUCUGc -3' miRNA: 3'- -ACCAGCCGCUCGCGCCAUGGCGAGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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