Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12447 | 3' | -61.6 | NC_003324.1 | + | 5573 | 0.66 | 0.412463 |
Target: 5'- -cCGCGGGCGUCa-UCGGccugACCGuGAUc -3' miRNA: 3'- uuGCGCCCGCGGgaAGCC----UGGCuCUG- -5' |
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12447 | 3' | -61.6 | NC_003324.1 | + | 42487 | 0.66 | 0.412463 |
Target: 5'- uGGCGCuGGCGCUgUUgCGGACCcGcGACc -3' miRNA: 3'- -UUGCGcCCGCGGgAA-GCCUGG-CuCUG- -5' |
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12447 | 3' | -61.6 | NC_003324.1 | + | 48797 | 0.66 | 0.412463 |
Target: 5'- cGACGCGguuGGCGCCCUUuucguauuucUGGACCugcuGGAa -3' miRNA: 3'- -UUGCGC---CCGCGGGAA----------GCCUGGc---UCUg -5' |
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12447 | 3' | -61.6 | NC_003324.1 | + | 38124 | 0.66 | 0.403532 |
Target: 5'- -uUGUGGG-GCgUUUCGGACC-AGACg -3' miRNA: 3'- uuGCGCCCgCGgGAAGCCUGGcUCUG- -5' |
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12447 | 3' | -61.6 | NC_003324.1 | + | 24870 | 0.67 | 0.360767 |
Target: 5'- -uCGCGGGCGgCCUcgUUGGAaccgUCGAGGa -3' miRNA: 3'- uuGCGCCCGCgGGA--AGCCU----GGCUCUg -5' |
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12447 | 3' | -61.6 | NC_003324.1 | + | 48511 | 0.67 | 0.360767 |
Target: 5'- cGGCGC-GGCGCUCUUCGa--UGAGGCg -3' miRNA: 3'- -UUGCGcCCGCGGGAAGCcugGCUCUG- -5' |
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12447 | 3' | -61.6 | NC_003324.1 | + | 6190 | 0.67 | 0.344568 |
Target: 5'- aGGCGCGGGUaacgacuucGCCaagUUGGACCGAcuugcccucGGCg -3' miRNA: 3'- -UUGCGCCCG---------CGGga-AGCCUGGCU---------CUG- -5' |
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12447 | 3' | -61.6 | NC_003324.1 | + | 43274 | 0.67 | 0.344568 |
Target: 5'- aGACGUGGGUGCgccuugCCUUCG--UCGAGGCu -3' miRNA: 3'- -UUGCGCCCGCG------GGAAGCcuGGCUCUG- -5' |
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12447 | 3' | -61.6 | NC_003324.1 | + | 42744 | 0.7 | 0.228196 |
Target: 5'- aAGCGUGGGCGCUCUcccaugcgcggaUCGGcaACgGAGAa -3' miRNA: 3'- -UUGCGCCCGCGGGA------------AGCC--UGgCUCUg -5' |
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12447 | 3' | -61.6 | NC_003324.1 | + | 20273 | 0.7 | 0.228196 |
Target: 5'- gGGCGCGuuacgcGCGCCUcUCuGACCGAGGCu -3' miRNA: 3'- -UUGCGCc-----CGCGGGaAGcCUGGCUCUG- -5' |
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12447 | 3' | -61.6 | NC_003324.1 | + | 11295 | 0.7 | 0.216934 |
Target: 5'- cACGgGGGuCGCCUcauuuggCGuGACCGAGGCg -3' miRNA: 3'- uUGCgCCC-GCGGGaa-----GC-CUGGCUCUG- -5' |
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12447 | 3' | -61.6 | NC_003324.1 | + | 38425 | 0.71 | 0.195835 |
Target: 5'- cAGCGCcgccccGGUGCCC-UCGuGAUCGAGACg -3' miRNA: 3'- -UUGCGc-----CCGCGGGaAGC-CUGGCUCUG- -5' |
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12447 | 3' | -61.6 | NC_003324.1 | + | 45858 | 0.72 | 0.158976 |
Target: 5'- cGGCGCgGGGCGCCUgaugCGaGGCCGuGAUg -3' miRNA: 3'- -UUGCG-CCCGCGGGaa--GC-CUGGCuCUG- -5' |
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12447 | 3' | -61.6 | NC_003324.1 | + | 584 | 0.76 | 0.074436 |
Target: 5'- gGGCGCGaacuGCGUCCgUUGGACCGAGGCg -3' miRNA: 3'- -UUGCGCc---CGCGGGaAGCCUGGCUCUG- -5' |
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12447 | 3' | -61.6 | NC_003324.1 | + | 28511 | 1.06 | 0.000448 |
Target: 5'- cAACGCGGGCGCCCUUCGGACCGAGACc -3' miRNA: 3'- -UUGCGCCCGCGGGAAGCCUGGCUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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