Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12449 | 3' | -56.7 | NC_003324.1 | + | 48755 | 0.71 | 0.372434 |
Target: 5'- -aGGugAUGCCGAGaugCG-CAGCCAGc -3' miRNA: 3'- ugCCugUGCGGCUUga-GCaGUCGGUC- -5' |
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12449 | 3' | -56.7 | NC_003324.1 | + | 44423 | 0.66 | 0.665421 |
Target: 5'- uGCGGGCaccggGCGCCGAGaCUUcgGUCgcugauGGCCGGc -3' miRNA: 3'- -UGCCUG-----UGCGGCUU-GAG--CAG------UCGGUC- -5' |
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12449 | 3' | -56.7 | NC_003324.1 | + | 43606 | 0.71 | 0.372434 |
Target: 5'- gGCGGuCGCGCuCGAcuGCUCGgacagcaAGCCGGa -3' miRNA: 3'- -UGCCuGUGCG-GCU--UGAGCag-----UCGGUC- -5' |
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12449 | 3' | -56.7 | NC_003324.1 | + | 43565 | 0.66 | 0.665421 |
Target: 5'- ---cACACGCCGcGCaaCGUCAGCCGa -3' miRNA: 3'- ugccUGUGCGGCuUGa-GCAGUCGGUc -5' |
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12449 | 3' | -56.7 | NC_003324.1 | + | 36715 | 0.71 | 0.347206 |
Target: 5'- uGCGGAUGCGCUGGcGCUgG-UAGCCAGu -3' miRNA: 3'- -UGCCUGUGCGGCU-UGAgCaGUCGGUC- -5' |
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12449 | 3' | -56.7 | NC_003324.1 | + | 35729 | 0.76 | 0.194732 |
Target: 5'- uGCGGAUACGCuucaCGAGCUCGUCGcGCUg- -3' miRNA: 3'- -UGCCUGUGCG----GCUUGAGCAGU-CGGuc -5' |
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12449 | 3' | -56.7 | NC_003324.1 | + | 35464 | 0.66 | 0.676197 |
Target: 5'- cGCGGGCG-GCCGAGCagGcugcagCAGCaCAGg -3' miRNA: 3'- -UGCCUGUgCGGCUUGagCa-----GUCG-GUC- -5' |
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12449 | 3' | -56.7 | NC_003324.1 | + | 33507 | 0.68 | 0.542751 |
Target: 5'- cACGGACaACGCCGAugaagcgGCUCGggcguUCAucgcaagcuucuccGCCAGu -3' miRNA: 3'- -UGCCUG-UGCGGCU-------UGAGC-----AGU--------------CGGUC- -5' |
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12449 | 3' | -56.7 | NC_003324.1 | + | 32895 | 0.73 | 0.293203 |
Target: 5'- gGCGGcugcccgccAUGCGCCGAacaGCUCGUagAGCCAGa -3' miRNA: 3'- -UGCC---------UGUGCGGCU---UGAGCAg-UCGGUC- -5' |
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12449 | 3' | -56.7 | NC_003324.1 | + | 29484 | 0.66 | 0.665421 |
Target: 5'- uGCGGGCGCGUCGGGCgcaucUCGguaacCGGCUu- -3' miRNA: 3'- -UGCCUGUGCGGCUUG-----AGCa----GUCGGuc -5' |
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12449 | 3' | -56.7 | NC_003324.1 | + | 29075 | 1.09 | 0.000851 |
Target: 5'- gACGGACACGCCGAACUCGUCAGCCAGa -3' miRNA: 3'- -UGCCUGUGCGGCUUGAGCAGUCGGUC- -5' |
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12449 | 3' | -56.7 | NC_003324.1 | + | 28145 | 0.7 | 0.445531 |
Target: 5'- gGCGGAUGCgGCCGAcCUUGUaguuGCCGGc -3' miRNA: 3'- -UGCCUGUG-CGGCUuGAGCAgu--CGGUC- -5' |
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12449 | 3' | -56.7 | NC_003324.1 | + | 27487 | 0.7 | 0.4265 |
Target: 5'- gGCGuGACGCaagGCCGGGCUUGaaccUCAGCgCAGg -3' miRNA: 3'- -UGC-CUGUG---CGGCUUGAGC----AGUCG-GUC- -5' |
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12449 | 3' | -56.7 | NC_003324.1 | + | 25311 | 0.68 | 0.546973 |
Target: 5'- gGCGGcCAggUGCuCGAAC-CGUCAGUCGGc -3' miRNA: 3'- -UGCCuGU--GCG-GCUUGaGCAGUCGGUC- -5' |
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12449 | 3' | -56.7 | NC_003324.1 | + | 24829 | 0.72 | 0.323224 |
Target: 5'- gGCGGAuCGCGCCGAcaagGUCGGCCGu -3' miRNA: 3'- -UGCCU-GUGCGGCUugagCAGUCGGUc -5' |
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12449 | 3' | -56.7 | NC_003324.1 | + | 24383 | 0.71 | 0.372434 |
Target: 5'- uACGGGCugGCCGAGaUgGUCGGgCAu -3' miRNA: 3'- -UGCCUGugCGGCUUgAgCAGUCgGUc -5' |
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12449 | 3' | -56.7 | NC_003324.1 | + | 23683 | 0.66 | 0.65894 |
Target: 5'- cACGG-CGCGCCGGGCUucacaucgaUGUCGacugccucgauguccGCCGGc -3' miRNA: 3'- -UGCCuGUGCGGCUUGA---------GCAGU---------------CGGUC- -5' |
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12449 | 3' | -56.7 | NC_003324.1 | + | 22697 | 0.75 | 0.205248 |
Target: 5'- aGCGcaucCACGCCGAGCUCGUCgaaGGUCAGc -3' miRNA: 3'- -UGCcu--GUGCGGCUUGAGCAG---UCGGUC- -5' |
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12449 | 3' | -56.7 | NC_003324.1 | + | 21308 | 0.7 | 0.40796 |
Target: 5'- gAUGGAaaCGCGCCGucgccuucggcAGCUCGaaAGCCAGg -3' miRNA: 3'- -UGCCU--GUGCGGC-----------UUGAGCagUCGGUC- -5' |
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12449 | 3' | -56.7 | NC_003324.1 | + | 18041 | 0.66 | 0.654615 |
Target: 5'- cUGGAagACGCacuucgacuauuCGAACUCGUCgcGGCCGGc -3' miRNA: 3'- uGCCUg-UGCG------------GCUUGAGCAG--UCGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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