miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12451 5' -53.8 NC_003324.1 + 19179 0.66 0.798545
Target:  5'- aGCGGUCcUCUGCGcGGCGcUGGgcGa-- -3'
miRNA:   3'- -CGCUAGuAGACGU-CCGC-ACCuuCgac -5'
12451 5' -53.8 NC_003324.1 + 36740 0.67 0.747513
Target:  5'- aGUGAcaUCAUCcgaUGCgAGGCccGUGGAGcGCUGg -3'
miRNA:   3'- -CGCU--AGUAG---ACG-UCCG--CACCUU-CGAC- -5'
12451 5' -53.8 NC_003324.1 + 47510 0.67 0.726171
Target:  5'- -aGAgCGUagugGCAGGCGUcGAAGCUGu -3'
miRNA:   3'- cgCUaGUAga--CGUCCGCAcCUUCGAC- -5'
12451 5' -53.8 NC_003324.1 + 2344 0.67 0.704444
Target:  5'- cGCGGUCG-CUGCGGGaGUGGuuGUc- -3'
miRNA:   3'- -CGCUAGUaGACGUCCgCACCuuCGac -5'
12451 5' -53.8 NC_003324.1 + 54015 0.69 0.582149
Target:  5'- gGCG-UCuuUCUGCAGGCG-GcGAAGCg- -3'
miRNA:   3'- -CGCuAGu-AGACGUCCGCaC-CUUCGac -5'
12451 5' -53.8 NC_003324.1 + 29827 1.1 0.001232
Target:  5'- gGCGAUCAUCUGCAGGCGUGGAAGCUGc -3'
miRNA:   3'- -CGCUAGUAGACGUCCGCACCUUCGAC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.