Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12452 | 3' | -53.2 | NC_003324.1 | + | 23606 | 0.66 | 0.853114 |
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Target: 5'- gCUGUGGggcacCGAUcGUGCCGGGGUUg -3' miRNA: 3'- gGGCGCCaga--GCUA-UACGGUUCCAAg -5' |
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| 12452 | 3' | -53.2 | NC_003324.1 | + | 11625 | 0.67 | 0.787501 |
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Target: 5'- gCCCGCaaaGGccgaaaUCUUGAUcgcaaccAUGCCAAGGUa- -3' miRNA: 3'- -GGGCG---CC------AGAGCUA-------UACGGUUCCAag -5' |
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| 12452 | 3' | -53.2 | NC_003324.1 | + | 34178 | 0.68 | 0.747708 |
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Target: 5'- uCCCaguuCGGUCUCGAa--GCCGAGGc-- -3' miRNA: 3'- -GGGc---GCCAGAGCUauaCGGUUCCaag -5' |
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| 12452 | 3' | -53.2 | NC_003324.1 | + | 12216 | 0.68 | 0.726557 |
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Target: 5'- uCUCGCgGGUCUCGcagagAUGCCGAauGGUa- -3' miRNA: 3'- -GGGCG-CCAGAGCua---UACGGUU--CCAag -5' |
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| 12452 | 3' | -53.2 | NC_003324.1 | + | 36131 | 0.68 | 0.705023 |
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Target: 5'- gCCCGauaCGGcgUUGGUAgugcgGCCAAGGUUCu -3' miRNA: 3'- -GGGC---GCCagAGCUAUa----CGGUUCCAAG- -5' |
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| 12452 | 3' | -53.2 | NC_003324.1 | + | 18846 | 0.69 | 0.694142 |
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Target: 5'- -aCGcCGGUCUCGAUcuUGCCAuuuGGgaagUCg -3' miRNA: 3'- ggGC-GCCAGAGCUAu-ACGGUu--CCa---AG- -5' |
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| 12452 | 3' | -53.2 | NC_003324.1 | + | 6913 | 0.69 | 0.661181 |
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Target: 5'- aCCGCGG--UCGGUA--UCAAGGUUCa -3' miRNA: 3'- gGGCGCCagAGCUAUacGGUUCCAAG- -5' |
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| 12452 | 3' | -53.2 | NC_003324.1 | + | 42235 | 0.7 | 0.605834 |
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Target: 5'- gCCCGcCGGUCU-GAUAgUGCuCAcGGUUCu -3' miRNA: 3'- -GGGC-GCCAGAgCUAU-ACG-GUuCCAAG- -5' |
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| 12452 | 3' | -53.2 | NC_003324.1 | + | 31560 | 1.12 | 0.001061 |
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Target: 5'- gCCCGCGGUCUCGAUAUGCCAAGGUUCg -3' miRNA: 3'- -GGGCGCCAGAGCUAUACGGUUCCAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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