Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12452 | 5' | -50.7 | NC_003324.1 | + | 36396 | 0.66 | 0.931065 |
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Target: 5'- cGGuGGAAGACA--UCA--ACGaGCUGGCg -3' miRNA: 3'- -CC-CUUUCUGUcuGGUauUGC-CGACCG- -5' |
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| 12452 | 5' | -50.7 | NC_003324.1 | + | 37987 | 0.72 | 0.644016 |
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Target: 5'- gGGGuuAGGCGG-CCAUGccggagcgguuGCGGCgcacggGGCg -3' miRNA: 3'- -CCCuuUCUGUCuGGUAU-----------UGCCGa-----CCG- -5' |
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| 12452 | 5' | -50.7 | NC_003324.1 | + | 38721 | 0.66 | 0.92526 |
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Target: 5'- cGGGucGAGACAuACCA-GGCGcGCUuGGCc -3' miRNA: 3'- -CCCu-UUCUGUcUGGUaUUGC-CGA-CCG- -5' |
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| 12452 | 5' | -50.7 | NC_003324.1 | + | 45845 | 0.7 | 0.764882 |
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Target: 5'- cGGAAGGcgugGCAGGCCAUGAuCGcuccGCUGGa -3' miRNA: 3'- cCCUUUC----UGUCUGGUAUU-GC----CGACCg -5' |
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| 12452 | 5' | -50.7 | NC_003324.1 | + | 49108 | 0.67 | 0.891905 |
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Target: 5'- cGGGAGAgGugAGAagCAUGGCcGCgagGGCg -3' miRNA: 3'- -CCCUUU-CugUCUg-GUAUUGcCGa--CCG- -5' |
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| 12452 | 5' | -50.7 | NC_003324.1 | + | 49832 | 0.66 | 0.931065 |
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Target: 5'- aGGuc--ACAGcUCGUGccGCGGCUGGCa -3' miRNA: 3'- cCCuuucUGUCuGGUAU--UGCCGACCG- -5' |
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| 12452 | 5' | -50.7 | NC_003324.1 | + | 55034 | 0.68 | 0.876595 |
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Target: 5'- ----cGGGCAGGCaaggcgGACGGCUGGg -3' miRNA: 3'- cccuuUCUGUCUGgua---UUGCCGACCg -5' |
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| 12452 | 5' | -50.7 | NC_003324.1 | + | 56131 | 0.66 | 0.941802 |
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Target: 5'- uGGAAGccGACGG-CCGUGGCGacuUUGGCg -3' miRNA: 3'- cCCUUU--CUGUCuGGUAUUGCc--GACCG- -5' |
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| 12452 | 5' | -50.7 | NC_003324.1 | + | 57045 | 0.66 | 0.92526 |
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Target: 5'- cGGGuuGGGCuggGGAgCG--GCGGCgagGGCg -3' miRNA: 3'- -CCCuuUCUG---UCUgGUauUGCCGa--CCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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