miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12453 3' -53.1 NC_003324.1 + 31663 0.9 0.036674
Target:  5'- gAGAAGUUgC-CGCCGUUCCAUCCGAGAu -3'
miRNA:   3'- -UCUUCAA-GcGCGGCAAGGUAGGCUCU- -5'
12453 3' -53.1 NC_003324.1 + 52770 0.69 0.66647
Target:  5'- cGAGGaUCGCGCCGgaucgaCCAUCCa--- -3'
miRNA:   3'- uCUUCaAGCGCGGCaa----GGUAGGcucu -5'
12453 3' -53.1 NC_003324.1 + 7779 0.69 0.699435
Target:  5'- ---cGUUCGCGCCGguguuUCCGGGGu -3'
miRNA:   3'- ucuuCAAGCGCGGCaagguAGGCUCU- -5'
12453 3' -53.1 NC_003324.1 + 29962 0.68 0.742401
Target:  5'- cGAAGaUCGCGCCGUaUCCAcCCu--- -3'
miRNA:   3'- uCUUCaAGCGCGGCA-AGGUaGGcucu -5'
12453 3' -53.1 NC_003324.1 + 17210 0.67 0.803214
Target:  5'- cGgcGUUCuugaGCGCCGagUUCCGcucauaggucUCCGAGAu -3'
miRNA:   3'- uCuuCAAG----CGCGGC--AAGGU----------AGGCUCU- -5'
12453 3' -53.1 NC_003324.1 + 21599 0.66 0.857497
Target:  5'- ------aUGCGCCGUggcggCCGUCCGAc- -3'
miRNA:   3'- ucuucaaGCGCGGCAa----GGUAGGCUcu -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.